Programming DNA-Based Biomolecular Reaction Networks on Cancer Cell Membranes
Programming DNA-Based Biomolecular Reaction Networks on Cancer Cell Membranes
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DOI：
10.1021/jacs.9b05598
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发表时间：
2019-10-23
影响因子：
15
通讯作者：
Reif, John
中科院分区：
文献类型：
--
作者：
Song, Tianqi;Shah, Shalin;Reif, John
DNA是一种高度可编程的生物分子，已被用于构建不同目的的生物电路。DNA电路的一个重要发展是处理细胞膜上受体的信息。在这篇通讯中，我们介绍了一种在癌细胞膜上编程局部DNA生物分子反应网络的体系结构。基于我们的体系结构，各种类型的反应网络已经被实验证明，从简单的线性级联到复杂结构的反应网络。这些基于DNA的局部反应网络可用于医疗应用，如癌细胞检测。与已有的用于评估细胞膜受体的DNA电路相比，该结构制作的DNA电路具有设计简单、泄漏少、成本低和信本底比高等主要优点。
DNA is a highly programmable biomolecule and has been used to construct biological circuits for different purposes. An important development of DNA circuits is to process the information on receptors on cell membranes. In this Communication, we introduce an architecture to program localized DNA-based biomolecular reaction networks on cancer cell membranes. Based on our architecture, various types of reaction networks have been experimentally demonstrated, from simple linear cascades to reaction networks of complex structures. These localized DNA-based reaction networks can be used for medical applications such as cancer cell detection. Compared to prior work on DNA circuits for evaluating cell membrane receptors, the DNA circuits made by our architecture have several major advantages including simpler design, lower leak, lower cost, and higher signal-to-background ratio.