Differences in leukocyte phenotype and interferon-γ expression in stroma and endothelium during corneal graft rejection
Differences in leukocyte phenotype and interferon-γ expression in stroma and endothelium during corneal graft rejection
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DOI：
10.1016/j.exer.2005.12.018
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发表时间：
2006-08-01
影响因子：
3.4
通讯作者：
Dick, Andrew D.
中科院分区：
文献类型：
--
作者：
Nicholls, Susan M.;Banerjee, Sanjiv;Dick, Andrew D.
人类角膜移植成功的关键是预防角膜内皮排斥，但人们对内皮浸润知之甚少。为了检查内皮，使用了将该层去除并加工成平板的方法，并将浸润物与基质和上皮进行比较。将LEW或PVG品系大鼠角膜移植至PVG品系受体。移植后通过裂隙灯监测临床变化，并在排斥期间的一系列时间点处死动物。第 10 天至第 15 天发生临床定义的排斥，并伴有上皮排斥线和内皮细胞浸润。在这些时间点，同种移植物的基质中有一些白细胞浸润，但同种异体移植物中明显更多（所有亚组的 p < 0.003）。任何时候都没有同种移植物内皮的浸润，第10天也没有同种异体移植物内皮的浸润。第15天，基质中除B细胞外的所有主要亚群的数量相似，而在内皮巨噬细胞上，MHC II(+)类细胞和CD(8+)细胞占主导地位(p < 0.001 CD4(+)与CD8(+)细胞)。 T细胞和NK细胞在上皮排斥线中占主导地位。虽然间质和上皮细胞中产生大量 TNF-α 和 IFN-γ 细胞，但在内皮细胞中未发现产生 IFN-γ 的细胞。角膜层内浸润轮廓的明显差异表明排斥机制也可能不同。有限的内皮细胞特征和缺乏 IFN-γ 表明抗内皮反应可能受到前房环境的调节。 (c) 2006 Elsevier Ltd. 保留所有权利。
Critical to the success of human corneal transplants is prevention of corneal endothelial rejection, yet little is known about the endothelial infiltrate. To examine the endothelium, a method for removal and processing this layer as a flat sheet was used and the infiltrate was compared with stroma and epithelium. LEW or PVG strain rat comeas were transplanted to PVG strain recipients., Clinical changes after transplantation were monitored by slit lamp and animals sacrificed at a range of time points during rejection. Clinically defined rejection, accompanied by an epithelial rejection line and endothelial cell infiltration, occurred between days 10 and 15. There was some infiltration of leukocytes in the stroma of isografts at these time points, but significantly more in allografts (p < 0.003 for all subsets). There was no infiltration of isograft endothelium at any time and no infiltration of allograft endothelium on day 10. On day 15, there were similar numbers of all major subsets except B cells in the stroma, while on the endothelium macrophages, MHC class II(+) cells and CD(8+) cells predominated (p < 0.001 CD4(+) vs CD8(+) cells). T cells and NK cells predominated in the epithelial rejection line. While TNF-alpha and IFN-gamma-producing cells were numerous in stroma and epithelium, no IFN-gamma-producing cells were found on endothelium. Distinct differences in infiltrative profile within layers of the cornea suggest that the mechanisms of rejection may also differ. The restricted endothelial cell profile and lack of IFN-gamma suggests that the anti-endothelial response may be modulated by the anterior chamber environment. (c) 2006 Elsevier Ltd. All rights reserved.