Identification of Cytochrome P450 (CYP) genes in Zhikong scallop (Chlamys farreri)
Identification of Cytochrome P450 (CYP) genes in Zhikong scallop (Chlamys farreri)
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栉孔扇贝细胞色素P450（CYP）基因的鉴定
DOI：
10.1007/s11802-013-1967-5
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发表时间：
2013-03-01
影响因子：
1.6
通讯作者：
Hu Xiaoli
中科院分区：
文献类型：
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作者：
Guo Huihui;Bao Zhenmin;Hu Xiaoli
细胞色素P450（Cytochrome P450，CYP）超家族是生物体内成员最多、功能最丰富的蛋白质超家族之一。该超家族的成员根据其氨基酸相似性被进一步分配到不同的家族和亚家族。根据它们的系统发育关系，可能从共同祖先基因分化出来并可能具有共同功能的基因被聚为一个家族。扇贝是分布最广的一类双壳类，但对其寄生虫的研究较少。本研究以栉孔扇贝（Chlamys farreri）为研究对象，对栉孔扇贝基因组和表达序列标签进行了搜索。总共鉴定出88个非冗余的SNP，它们归巢于13个CYP基因家族。系统发育分析将这些基因分为4个家族。与后口类一样，第2族的基因数最多，共包含33个基因，分别属于CYP 1、CYP 2、CYP 17和CYP 356家族。第3家族包含19个基因，分别属于CYP3、CYP5和CYP30家族。家族4含有23个基因，均属于CYP 4家族。线粒体CYP10和CYP24家族共9个基因。相比之下，原口类（C. farreri、黑腹拟步行虫D. pluex、黑腹拟步行虫D. melanogaster）比后口类（S.而在第3、4族中基因较少。我们的研究结果将有助于解释扇贝和双壳类的功能和进化。
Cytochrome P450 (CYP) superfamily is one of the membership largest and function most diverse protein superfamily recogniozed among living beings. Members of this superfamily were further assigned to different families and subfamilies based on their amino acid similarities. According to their phylogenetic relationships, the CYP genes which likely diverged from common ancestor gene and may share common functions were grouped into one clan. Widely distributing scallops are a group of the most conspicuous bivalve; however the studies on their CYP is acarce. In this study, we searched the genome and expressed sequence tags of Zhikong scallop (Chlamys farreri) for CYP genes. In total, 88 non-redundant CYP were identified, which were homed in 13 CYPs gene families. Phylogenetic analysis divided these genes into 4 CYP clans. As in deuterostomes, Clan 2 was the largest, which contained 33 genes belonging to CYP1, CYP2, CYP17 and CYP356 families. Clan 3 contgained 19 genes belonging to CYP3, CYP5 and CYP30 families. Clan 4 contained 23 genes, all belonging to CYP4 family. The mitochondrial CYP clan contained 9 genes belonging to CYP10 and CYP24 families. In comparison, protostomes (C. farreri, D. pluex, D. melanogaster) contained more CYP genes than deuterostomes (S. purpuratus and vertebrates) in Clan 2 but less genes in Clan 3 and Clan 4. Our findings will aid to deciphering CYP function and evolution in scallops and bivalves.