Primary contribution of the injector to carryover of a trace analyte in high-performance liquid chromatography.
Primary contribution of the injector to carryover of a trace analyte in high-performance liquid chromatography.
复制标题
进样器对高效液相色谱中痕量分析物残留的主要贡献。
DOI：
10.1016/0021-9673(93)80515-a
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Giese,RW
中科院分区：
文献类型：
--
作者：
Saha,M;Giese,RW
低纳克量的N1， n3 -二-（五氟苯）- n7 -（2-[五氟苯氧基]乙基）黄嘌呤经高效液相色谱检测后，在注入纯流动相后（离线气相色谱-电子捕获负离子质谱法）可以检测到低纳克量的化合物。尽管对进样器和色谱柱进行了大量的中间洗涤。经测定，99.9%的分析物污染基本上来自注射器。使用两个喷射器是解决这个问题的一种切实可行的方法。
When a low-nanogram amount of N1,N3-bis-(pentafluorobenzyl)-N7-(2-[pentafluorobenzyloxy]ethyl) xanthine was subjected to HPLC, low-picogram amounts of the compound could be detected subsequently (off-line by gas chromatography—electron-capture negative-ion mass spectrometry) after injection of pure mobile phase. This was in spite of significant, intermediate washing of the injector and column. It was determined that essentially 99.9% of this analyte contamination came from the injector. Use of two injectors is a practical remedy for this problem.