Dimethyl Sulfoxide As a Protective Agent During Freezing and Thawing of Human Spermatozoa.∗
Dimethyl Sulfoxide As a Protective Agent During Freezing and Thawing of Human Spermatozoa.∗
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二甲亚砜作为人类精子冷冻和解冻过程中的保护剂。*
DOI：
10.3181/00379727-117-29552
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发表时间：
1964
影响因子：
--
通讯作者：
J. K. Sherman
中科院分区：
文献类型：
--
作者：
J. K. Sherman
摘要对二甲基亚砜(DMSO)作为人类精子冻融保护剂的活力进行了评价。在这种保护能力上，DMSO被发现与甘油相当，但在22°C的冷冻前和解冻后的储存过程中，DMSO的毒性都更大。在5%、10%、15%和20%DMSO的比较中发现了保护和毒性效应的交互作用，这表明10%的水平是最有利的。根据研究结果，在用所描述的方法冷冻保存人类精子时，不推荐使用DMSO作为甘油的替代品。我们衷心感谢金京秀先生的卓越技术援助，以及16名捐献精液的医科学生的合作。
Summary Dimethyl sulfoxide (DMSO) was evaluated, in terms of motility, as a freeze-thaw protective agent with human spermatozoa. DMSO was found to be equal to glycerol in this protective capacity but proved more toxic during storage at 22°C both before freezing and after thawing. An interaction of protective and toxic effects was noted in a comparison of 5, 10, 15 and 20% DMSO which suggested the 10% level as the most favorable. On the basis of findings, DMSO is not recommended as a substitute for glycerol in the preservation of human spermatozoa by freezing with the methods described. The superior technical assistance of Mr. Kyung Soo Kim is gratefully acknowledged, as is the cooperation of 16 medical students who donated semen.