Microstructure and cytocompatibility of electrospun nanocomposites based on poly(ε-caprolactone) and carbon nanostructures
Microstructure and cytocompatibility of electrospun nanocomposites based on poly(ε-caprolactone) and carbon nanostructures
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DOI：
10.1177/039139881003300502
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发表时间：
2010-05-01
影响因子：
1.7
通讯作者：
Folin, Marcella
中科院分区：
文献类型：
--
作者：
Bianco, Alessandra;Del Gaudio, Costantino;Folin, Marcella
碳纳米结构（CNS）对于下一代组织工程支架（尤其是神经假体）来说是有吸引力且有前途的纳米材料。优化支架血管化可能是促进受损脑组织修复的重要策略。在这种情况下，我们的想法是评估负载中枢神经系统的电纺纳米混合支架的细胞反应。采用静电纺丝技术制备了基于聚（ε-己内酯）（PCL）和 CNS 的纤维复合材料。研究了高纯度碳纳米纤维（CNF）和单壁碳纳米管（SWNT）。进行了详细的微观结构表征，以评估实现纤维 PCL/CNS 织物的最有利的实验条件。从四氢呋喃 (THF) 和 N,N 二甲基甲酰胺 (DMF) 的 1:1 v/v 混合物开始，获得由相当均匀且同质的亚微米纤维组成的电纺垫。使用大鼠脑微血管内皮细胞（CEC）进行体外细胞相容性测试。获得的结果显示 PCL/CNS 纳米复合材料的细胞活力增加，表明这些材料是内皮细胞的合适环境。这些结果表明基于中枢神经系统的纳米复合材料在需要内皮功能特性的组织工程应用的生物医学设备中具有广阔的前景。
Carbon nanostructures (CNSs) are attractive and promising nanomaterials for the next generation of tissue engineering scaffolds, especially in neural prosthesis. Optimizing scaffold vascularization may be an important strategy to promote the repair of damaged brain tissue. In this context, the idea was to evaluate the cell response of electrospun nanohybrid scaffolds loaded with CNSs. Fibrous composites based on poly(epsilon-caprolactone) (PCL) and CNSs were fabricated by means of electrospinning technique. High-purity carbon nanofibers (CNFs) and single-wall carbon nanotubes (SWNTs) were studied. A detailed microstructural characterization was performed to evaluate the most favorable experimental conditions for the realization of fibrous PCL/CNS fabrics. Electrospun mats comprised of rather uniform and homogeneous submicrometric fibers were obtained starting from 1:1 v/v mixture of tetrahydrofuran (THF) and N,N dimethylformamide (DMF). In vitro cytocompatibility tests were performed using rat cerebro-microvascular endothelial cells (CECs). Acquired results showed an increased cell viability for PCL/CNS nanocomposites, suggesting these materials as a suitable environment for endothelial cells. These results are indicative of the promising potential of CNS-based nanocomposites in biomedical devices for tissue engineering applications where endothelial functional properties are required.