Flagellar adhesion and deadhesion in Chlamydomonas gametes: effects of tunicamycin and observations on flagellar tip morphology.
Flagellar adhesion and deadhesion in Chlamydomonas gametes: effects of tunicamycin and observations on flagellar tip morphology.
复制标题
衣藻配子中的鞭毛粘附和死粘附：衣霉素的影响和对鞭毛尖端形态的观察。
DOI：
10.1002/jsscb.1981.380160407
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Snell,WK
中科院分区：
文献类型：
--
作者：
Snell,WK
莱茵衣藻中鞭毛粘附性的聚集依赖性丧失与粘附和死粘附过程中鞭毛尖端形态的变化相关。当聚集的 mt− 和无能（能够粘附，但不能融合）的 mt+配子在蛋白质合成抑制剂放线菌酮存在下开始解聚时，鞭毛尖端形态也随之发生变化，从激活的球状形式变为非激活的锥形。在聚集过程中维持鞭毛粘附性所需的蛋白质合成活性可能部分是由于参与激活鞭毛尖端形成或稳定的蛋白质的周转。用衣霉素孵育聚集的配子表明，与蛋白质合成抑制剂一样，这种糖基化抑制剂也会导致粘附的配子死亡。结果表明，蛋白质糖基化可能对于维持聚集过程中的粘附性至关重要。
The aggregation‐dependent loss of flagellar adhesiveness in Chlamydomonas reinhardi has been correlated with changes in flagellar tip morphology during adhesion and deadhesion. As aggregating mt−and impotent (able to adhere, but not fuse) mt+gametes begin to disaggregate in the presence of the protein synthesis inhibitor cycloheximide, there is a concomitant change in flagellar tip morphology from the activated bulbous form to the nonactivated tapered shape. The requirement of protein‐synthetic activity for the maintenance of flagellar adhesiveness during aggregation may be due in part to turnover of proteins involved in formation or stabilization of activated flagellar tips.Incubation of aggregating gametes with tunicamycin indicates that, like protein synthesis inhibitors, this inhibitor of glycosylation also causes adhering gametes to deadhere. The results suggest that protein glycosylation may be essential for maintenance of adhesiveness during aggregation.