Co-localization of androgen receptor with estrogen receptor β in the lower urinary tract of the male rat
Co-localization of androgen receptor with estrogen receptor β in the lower urinary tract of the male rat
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DOI：
10.1016/s0022-5347(05)66041-7
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发表时间：
2001-08-01
影响因子：
6.6
通讯作者：
Mäkelä, S
中科院分区：
文献类型：
--
作者：
Salmi, S;Santti, R;Mäkelä, S
目的：雄激素和雌激素影响排尿。在本研究中，确定了它们在雄性大鼠下尿路的靶位点。 材料和方法：用特异性雌激素受体 tr、雌激素受体 β (ER β) 或雄激素受体 (AR) 抗体对成年雄性大鼠的膀胱体、膀胱颈、前列腺尿道、中近端尿道和前列腺自主神经节的冷冻切片进行免疫染色。然后在常规荧光或共焦荧光显微镜下检查切片。结果：尿路上皮、膀胱平滑肌细胞、近端尿道横纹肌细胞和前列腺丛自主神经节神经元中 AR 和 ER β 的 Go 表达表明雌激素和雄激素对下尿路有直接影响。结论：AR 和 ER β 在排尿激素控制中的局部相互作用是一种有趣的可能性。
Purpose: Androgens and estrogens influence voiding. In this study their target sites in the lower urinary tract of the male rat were identified.Materials and Methods: Cryosections of the bladder body, bladder neck, prostatic urethra, mid proximal urethra and prostatic autonomic ganglia of adult male rats were immunostained with specific estrogen receptor tr, estrogen receptor beta (ER beta) or androgen receptor (AR) antibodies. The sections were then examined under conventional, fluorescence or confocal fluorescence microscopy.Results: Go-expression of AR and ER beta in the urothelium, bladder smooth muscle cells, proximal urethra striated muscle cells and neurons in the autonomic ganglia of the prostatic plexus suggests that estrogen and androgen have direct effects in the lower urinary tract.Conclusions: The local interaction of AR and ER beta in the hormonal control of voiding is an intriguing possibility.