IN-SITU CHARACTERIZATION OF THE LATE VACUOLATE MICROSPORE AS A CONVENIENT STAGE TO INDUCE EMBRYOGENESIS IN CAPSICUM
IN-SITU CHARACTERIZATION OF THE LATE VACUOLATE MICROSPORE AS A CONVENIENT STAGE TO INDUCE EMBRYOGENESIS IN CAPSICUM
复制标题
DOI：
10.1007/bf01280233
复制
发表时间：
1995-01-01
期刊：
影响因子：
2.9
通讯作者：
RISUENO, MC
中科院分区：
文献类型：
--
作者：
GONZALEZMELENDI, P;TESTILLANO, PS;RISUENO, MC
辣椒花粉胚胎发生的诱导，选择液泡化后期的小孢子作为诱导的适宜发育时期。各种现代原位方法，包括细胞化学，免疫细胞化学和原位杂交已被用来表征的功能组织的小孢子核在这个特定的发展阶段，其中的变化，孢子体程序可以被诱导。染色质模式，染色质间结构和核仁亚室的结果显示，在后期液泡小孢子转录非常活跃的核，并定义这个特定时期的细胞特征。还对小孢子胚的第一阶段进行了研究，确定了二细胞和多细胞花粉粒核的主要超微结构特征。所使用的方法，提供信息的亚细胞位置和表达时间的关键分子参与基因表达，提出了一个很高的潜力，研究复杂的过程中的花粉胚胎发生诱导。
Pollen embryogenesis has been induced in Capsicum annuum L., selecting the late vacuolate microspore as the suitable developmental stage for the induction. Various modem in situ methods including cytochemistry, immunocytochemistry, and in situ hybridization have been used to characterize the functional organization of the microspore nucleus at this specific stage of development in which the change to the sporophytic program can be induced. The results on the chromatin pattern, interchromatin structures and nucleolar subcompartments reveal a transcriptionally very active nucleus in the late vacuolate microspore and define characteristic cellular features of this specific period. The first stages of the microspore derived embryos have also been studied identifying the main ultrastructural features exhibited by the nuclei of two-cell and multicellular pollen grains. The approach used, providing information on the subcellular location and expression time of key molecules involved in gene expression, presents a high potential for studying the complex process of pollen embryogenesis induction.