Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro
Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro
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DOI：
10.1016/j.joca.2004.10.015
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发表时间：
2005-02-01
影响因子：
7
通讯作者：
Freed, LE
中科院分区：
文献类型：
--
作者：
Tognana, E;Chen, F;Freed, LE
目的：通过体外模型验证培养时间和邻近组织结构组成对工程软骨软骨形成和整体修复的影响。方法：将牛软骨细胞和透明质酸苄酯无纺布网制成的工程构建物压入相邻的由关节软骨（AC）、活骨（DB）或活骨（VB）制成的组织环中，在旋转生物反应器中培养8周。评估其结构（光镜和电镜）、生物力学性能（界面粘附强度、结构压缩模量）、生化成分（结构糖胺聚糖（GAG）、胶原蛋白和细胞）和邻近组织扩散率。结果：工程构建体主要由透明软骨组成，由于细胞外基质的界面沉积，它们要么与相邻软骨紧密相对，要么与相邻骨功能交叉。延长培养时间可显著提高构建体黏附强度（P < 0.001）、模量（P = 0.02）、GAG （P = 0.04）和细胞体积（P < 0.001）。邻近组织类型显著影响构建体粘连（P < 0.001）、模量（P < 0.001）、GAG （P < 0.001）和胶原蛋白（P < 0.001）。对于在软骨环中培养的构建体，环GAG含量（对数变换后）与构建体粘附力（R-2 = 0.66, P < 0.005）、模量（R-2 = 0.49, P < 0.05）和GAG （R-2 = 0.44, P < 0.05）呈负相关。与骨相邻培养的构建体整体修复效果优于软骨，这与其坚实的建筑结构和高GAG含量有关；与DB相邻培养的构建体整体修复效果最好，与其高弥漫性有关。结论：工程软骨的软骨发生和综合修复随着时间的推移而改善，并取决于邻近组织的结构、组成和运输特性。(C) 2004国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objective: An in vitro model was used to test the hypothesis that culture time and adjacent tissue structure and composition affected chondrogenesis and integrative repair in engineered cartilage.Method: Engineered constructs made of bovine calf chondrocytes and hyaluronan benzyl ester non-woven mesh were press-fitted into adjacent tissue rings made of articular cartilage (AC), devitalized bone (DB), or vital bone (VB) and cultured in rotating bioreactors for up to 8 weeks. Structure (light and electron microscopy), biomechanical properties (interfacial adhesive strength, construct compressive modulus), biochemical composition (construct glycosaminoglycans (GAG), collagen, and cells), and adjacent tissue diffusivity were assessed.Results: Engineered constructs were comprised predominately of hyaline cartilage, and appeared either closely apposed to adjacent cartilage or functionally interdigitated with adjacent bone due to interfacial deposition of extracellular matrix. An increase in culture time significantly improved construct adhesive strength (P < 0.001), modulus (P = 0.02), GAG (P = 0.04) and cellularity (P < 0.001). The type of adjacent tissue significantly affected construct adhesion (P < 0.001), modulus (P < 0.001), GAG (P < 0.001) and collagen (P < 0.001). For constructs cultured in rings of cartilage, negative correlations were observed between ring GAG content (log transformed) and construct adhesion (R-2 = 0.66, P < 0.005), modulus (R-2 = 0.49, P < 0.05) and GAG (R-2 = 0.44, P < 0.05). Integrative repair was better for constructs cultured adjacent to bone than cartilage, in association with its solid architectural structure and high GAG content, and best for constructs cultured adjacent to DB, in association with its high diffusivity.Conclusions: Chondrogenesis and integrative repair in engineered cartilage improved with time and depended on adjacent tissue architecture, composition, and transport properties. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.