Conjugation-Induced Fluorescent Labeling of Proteins and Polymers Using Dithiomaleimides
Conjugation-Induced Fluorescent Labeling of Proteins and Polymers Using Dithiomaleimides
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DOI：
10.1021/ja3105494
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发表时间：
2013-02-27
影响因子：
15
通讯作者：
O'Reilly, Rachel K.
中科院分区：
文献类型：
--
作者：
Robin, Mathew P.;Wilson, Paul;O'Reilly, Rachel K.
具有烷基取代基的二硫代马来酰亚胺（DTM）是一类新型的高发射荧光团。通过选择N和S取代基，可以容易地定制可变的溶解度和进一步的官能化。将DTM单元包含在ROP/RAFT引发剂中或插入鲑鱼降钙素（sCT）的二硫键中证明了用于聚合物和蛋白质的荧光标记的实用性。同时PEG化和荧光标记的sCT也被证明，使用DTM单元作为连接体和荧光团。预计DTMs将提供一个有吸引力的替代常用的笨重，平面荧光团。
Dithiomaleimides (DTMs) with alkyl substituents are shown to be a novel class of highly emissive fluorophores. Variable solubility and further functionalization can easily be tailored through the choice of N and S substituents. Inclusion of a DTM unit into a ROP/RAFT initiator or insertion into the disulfide bond of salmon calcitonin (sCT) demonstrates the utility for fluorescent labeling of polymers and proteins. Simultaneous PEGylation and fluorescent labeling of sCT is also demonstrated, using the DTM unit as both a linker and a fluorophore. It is anticipated that DTMs will offer an attractive alternative to commonly used bulky, planar fluorophores.