DNA barcoding of stygofauna uncovers cryptic amphipod diversity in a calcrete aquifer in Western Australia's arid zone
DNA barcoding of stygofauna uncovers cryptic amphipod diversity in a calcrete aquifer in Western Australia's arid zone
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DOI：
10.1111/j.1755-0998.2009.02706.x
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发表时间：
2010-01-01
影响因子：
7.7
通讯作者：
Cooper, S. J. B.
中科院分区：
文献类型：
--
作者：
Bradford, T.;Adams, M.;Cooper, S. J. B.
西澳大利亚州干旱的Yilgarn地区含有许多地下钙质结含水层，其中有独特的专性地下水无脊椎动物（stygofauna）组合。我们的目标是为单个钙质结砾岩中存在的大型无脊椎动物建立一个DNA条形码框架，作为未来评估耶尔加恩钙质结砾岩生物多样性和研究食物网的基础。在斯特草甸钙质结砾岩的钻孔场网格进行了密集的采样，以获得整个大型无脊椎动物生态系统的代表。线粒体细胞色素c氧化酶1（COI）基因的一个623 bp的片段被用来提供的stygobiont大型无脊椎动物和陆地生物的钙质结层中发现的DNA条形码。系统发育分析表明，存在12个不同的单倍型单系群。地下端足类（Chiltoniidae）的COI单倍型有3组，它们之间的序列差异均大于11%。等位酶分析发现了大量的固定等位基因之间的差异，这三个端足类动物组，表明有三个形态上的神秘物种内的斯特草甸钙质结砾岩。不像现在的双齿甲虫的姐妹三胞胎，片脚类不是姐妹分支，并且与其他Yilgarn和非Yilgarn片脚类的关系比彼此更密切。我们的研究结果表明，含水层包含至少12个大型无脊椎动物物种和DNA条形码提供了一个有用的手段，在这个系统中区分物种。
The arid Yilgarn region of Western Australia contains numerous subterranean calcrete aquifers with unique assemblages of obligate groundwater invertebrates (stygofauna). We aimed to establish a DNA barcoding framework for the macro-invertebrates present in a single calcrete, as a basis for future assessment of biodiversity of the Yilgarn calcretes and for investigating food webs. Intense sampling of a bore field grid in the Sturt Meadows calcrete was undertaken to obtain representatives of the entire macro-invertebrate ecosystem. A 623-bp fragment of the mitochondrial cytochrome c oxidase 1 (COI) gene was used to provide DNA barcodes for stygobiont macro-invertebrates plus terrestrial organisms that are found in the calcrete. Phylogenetic analyses revealed the existence of 12 divergent monophyletic groups of haplotypes. Subterranean amphipods (Chiltoniidae) showed three groups of COI haplotypes with sequence divergences between them of > 11%. Allozyme analyses found a large number of fixed allelic differences between these three amphipod groups, indicating that there are three morphologically cryptic species within the Sturt Meadows calcrete. Unlike the sister triplet of dytiscid beetles present, the amphipods are not sister clades and are more closely related to other Yilgarn and non-Yilgarn amphipods than to each other. Our results show that the aquifer contains at least 12 macro-invertebrate species and DNA barcoding provides a useful means for discriminating species in this system.