Effects of a self-assembling peptide as a scaffold on bone formation in a defect.
Effects of a self-assembling peptide as a scaffold on bone formation in a defect.
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DOI：
10.1371/journal.pone.0190833
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ishiguro N
中科院分区：
文献类型：
--
作者：
Ando K;Imagama S;Kobayashi K;Ito K;Tsushima M;Morozumi M;Tanaka S;Machino M;Ota K;Nishida K;Nishida Y;Ishiguro N
脊柱融合和受伤后的骨缺损，骨肿瘤切除和感染需要通过植入修复。在老龄化社会中，从这些程序中恢复往往是困难的。在这项研究中，我们发现注射SPG-178导致几种骨标记基因的表达和体外矿化，并揭示了使用SPG-178体内新形成的骨基质的显著更高程度。MC 3 T3-E1细胞用于评价SPG-178促进的成骨细胞分化。为了分析基因表达，从用对照培养基或SPG-178培养基培养7天和14天的MC 3 T3-E1细胞中分离总RNA。在检查的几种骨标记基因中，SPG-178显著增加ALP、BMP-2和骨钙素、OPN、BSP和Osterix的mRNA水平。10周龄雌性Wistar大鼠用于所有移植程序。将PEEK椎间融合器植入左侧股骨中段的骨缺损（5 mm）中，并通过外固定器保持稳定性。PEEK融合器填充有SPG-178水凝胶加同种异体骨碎片（n = 4）或仅同种异体骨碎片（n = 4）。然后将大鼠饲养56天。PEEK椎间融合器内显示新形成的骨基质，与对照组相比，使用SPG-178填充的椎间融合器的每总体积的骨体积增加。SPG-178具有临床应用潜力，因为它有几个好处。这些包括其良好的骨传导特性，其作为各种不同细胞和生长因子的支持的能力，与动物来源的材料（如ECM）相比，其缺乏感染风险，以及其可用于填充具有复杂形状的缺损并与各种其他材料组合的容易性。
Spinal fusion and bone defect after injuries, removal of bone tumors, and infections need to be repaired by implantation. In an aging society, recovery from these procedures is often difficult. In this study, we found that injection of SPG-178 leads to expression of several bone marker genes and mineralization in vitro, and revealed a significantly higher degree of newly formed bone matrix with use of SPG-178 in vivo. MC3T3-E1 cells were used to evaluate osteoblast differentiation promoted by SPG-178. To analyze gene expression, total RNA was isolated from MC3T3-E1 cells cultured for 7 and 14 days with control medium or SPG-178 medium. Among the several bone marker genes examined, SPG-178 significantly increased the mRNA levels for ALP, BMP-2 and Osteocalcin, OPN, BSP and for the Osterix. Ten-week-old female Wistar rats were used for all transplantation procedures. A PEEK cage was implanted into a bony defect (5 mm) within the left femoral mid-shaft, and stability was maintained by an external fixator. The PEEK cages were filled with either a SPG-178 hydrogel plus allogeneic bone chips (n = 4) or only allogeneic bone chips (n = 4). The rats were then kept for 56 days. Newly formed bone matrix was revealed inside the PEEK cage and there was an increased bone volume per total volume with the cage filled with SPG-178, compared to the control group. SPG-178 has potential in clinical applications because it has several benefits. These include its favorable bone conduction properties its ability to act as a support for various different cells and growth factors, its lack of infection risk compared with materials of animal origin such as ECM, and the ease with which it can be used to fill defects with complex shapes and combined with a wide range of other materials.
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影响因子：
4.8
作者：
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通讯作者：
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