A significant enhancement of therapeutic effect against hepatic metastases of M5076 in mice by a liposomal interleukin-2 (mixture)
A significant enhancement of therapeutic effect against hepatic metastases of M5076 in mice by a liposomal interleukin-2 (mixture)
复制标题
DOI：
10.1016/s0168-3659(02)00083-4
复制
发表时间：
2002-08-21
影响因子：
10.8
通讯作者：
Hirano, K
中科院分区：
文献类型：
--
作者：
Kanaoka, E;Takahashi, K;Hirano, K
在最佳条件下（脂质体：DSPC-DSPG;摩尔比，10：1;尺寸为30-50 nm，IL-2与脂质体的比例：4.0 JRU/nmol脂质），重组白细胞介素-2（IL-2）被强烈且几乎完全吸附到小疏水脂质体上。该脂质体IL-2改善了IL-2在静脉内给药后的分布，如先前所报道的。脂质体IL-2（300-10 000 JRU/小鼠/天）在抑制小鼠中M5076的实验转移方面比单独的游离IL-2显著更有效。脂质体IL-2的抑制作用在肝脏中最大。脂质体IL-2和游离IL-2在肝脏中的ED_（50）分别为1640和12500 JRU/小鼠/d。这种使用IL-2和脂质体混悬液的简单制剂（混合物）预期具有增加针对肝转移的治疗功效的潜力。(C)2002 Elsevier Science BV保留所有权利。
Recombinant interleukin-2 (IL-2) was strongly and almost completely adsorbed onto small hydrophobic liposomes under optimal conditions (liposome: DSPC-DSPG; molar ratio, 10: 1; 30-50 nm in size, ratio of IL-2 to liposome: 4.0 JRU/nmol lipid). This liposomal IL-2 improved the distribution of IL-2 after intravenous administration as reported, previously. Liposomal IL-2 (300-10 000 JRU/mouse per day) was significantly more effective than free IL-2 alone for inhibiting against the experimental metastases of M5076 in mice. The inhibitory effect of liposomal IL-2 was greatest in the liver. The ED50 of liposomal IL-2 and that of free IL-2 in the liver were 1640 and 12 500 JRU/mouse per day, respectively. This simple preparation (mixture) using IL-2 and liposome suspension is expected to have potential for increasing therapeutic efficacy against hepatic metastases. (C) 2002 Elsevier Science BV All rights reserved.