Early effects of glucose and oxygen deprivation on the spontaneous acetylcholine release from the myenteric plexus of the guinea pig ileum
Early effects of glucose and oxygen deprivation on the spontaneous acetylcholine release from the myenteric plexus of the guinea pig ileum
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葡萄糖和缺氧对豚鼠回肠肌间神经丛自发乙酰胆碱释放的早期影响
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
H. R. Martins
中科院分区：
文献类型：
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作者：
R. Larson;H. R. Martins
葡萄糖和氧气剥夺对豚鼠回肠肌间神经丛自发乙酰胆碱输出的早期影响——豚鼠回肠的纵向肌肉准备，使用允许以 2 分钟间隔快速采集样本的培养室进行了研究。葡萄糖剥夺或缺氧导致自发乙酰胆碱输出率逐渐下降。然而，葡萄糖剥夺加上缺氧会导致乙酰胆碱输出在 10-15 分钟内加速，其速度比正常条件下观察到的速度快七倍。通过将氧气和葡萄糖重新引入浴液介质中来恢复低静息率。吗啡（2.7 × 10−5 M）和河豚毒素（1.6 × 10−6 M）都不能阻止能量剥夺引起的乙酰胆碱输出的增加。在EGTA存在下，用Mg2+替代Ca2+，大大减少了能量剥夺引起的乙酰胆碱输出。然而，在 t 下观察到少量短暂的乙酰胆碱输出。
The early effects of glucose and oxygen deprivation on the spontaneous acetylcholine output from the myenteric plexus – longitudinal muscle preparation of the guinea pig ileum were studied using an incubation chamber that permitted rapid sample collection in 2-min intervals. Glucose deprivation or hypoxia resulted in a gradual decline in rate of spontaneous acetylcholine output. However, glucose deprivation plus hypoxia caused an acceleration in acetylcholine output within 10–15 min, which attained a rate seven times greater than observed under normal conditions. Recovery of low resting rates was obtained by reintroduction of oxygen and glucose into the bath medium. Neither morphine (2.7 × 10−5 M) nor tetrodotoxin (1.6 × 10−6 M) prevented the increase in acetylcholine output induced by energy deprivation. The substitution of Ca2+ by Mg2+, in the presence of EGTA, greatly reduced the acetylcholine output induced by energy deprivation. However, a small transitory output of acetylcholine was observed under t...