Identification of β1-3 galactosylglucose-core free-glycans in human urine
Identification of β1-3 galactosylglucose-core free-glycans in human urine
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人尿液中 β1-3 半乳糖基葡萄糖核心游离聚糖的鉴定
DOI：
10.1016/j.ab.2021.114427
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发表时间：
2022
影响因子：
2.9
通讯作者：
Miyamoto Yasuhide
中科院分区：
文献类型：
--
作者：
Tanaka-Okamoto Miki;Hanzawa Ken;Murakami Hiroko;Mukai Mikio;Miyamoto Yasuhide
我们先前报道了人尿液中酸性游离聚糖的精确结构。在本研究中，对尿液中的中性游离聚糖进行了详细的结构分析。从21名健康志愿者中采集尿样，并从肌酐调整的尿液中提取游离聚糖，然后用2-氨基吡啶进行荧光标记。中性聚糖分析通过高效液相色谱法、质谱法、酶消化和高碘酸盐裂解的组合实现。共鉴定了79种聚糖。由于含有尿中性聚糖的ABO血型抗原是主要成分，因此相同ABO血型个体之间的分析模式相似。中性聚糖由乳糖核心（Galβ1-4Glc）聚糖、IIN型乙酰乳糖胺核心（GlcNAcβ1-4Glc）聚糖、己糖低聚物、N-聚糖和我们惊讶的β1-3半乳糖基葡萄糖核心（Galβ1-3Glc）聚糖组成。尽管具有β1-3半乳糖基葡萄糖核心的聚糖被鉴定为尿液中的主要组分，包括乳糖核心的结构简单异构体，但先前尚未报告核心结构。主要的β1-3半乳糖基葡萄糖核心聚糖为Fucα1-2Galβ1-3（Fucα1-4）Glc、GalNAcα1-3（Fucα1-2）Galβ1-3（Fucα1-4）Glc和Galα1-3（Fucα1-2）Galβ1-3（Fucα1-4）Glc，分别对应于H-、A-和B-血型抗原。还检测到三种乳糖胺延伸的β1-3半乳糖基葡萄糖核心聚糖作为次要组分。阐明β1-3半乳糖基葡萄糖的生物合成对于理解这些聚糖的体内功能至关重要。
We previously reported the precise structure of acidic free-glycans in human urine. In the present study, structural analysis of neutral free-glycans in urine was performed in fine detail. Urine samples were collected from 21 healthy volunteers and free-glycans extracted from the creatinine-adjusted urine and then fluorescently labeled with 2-aminopyridine. Neutral glycan profiling was achieved by a combination of high-performance liquid chromatography, mass spectrometry, enzymatic digestion, and periodate cleavage. A total of 79 glycans were identified. Because the ABO-blood group antigen containing urinary neutral glycans are major components, profiling patterns were similar between individuals of the same ABO-group. The neutral glycans were composed of lactose-core (Galβ1-4Glc) glycans, type-IIN-acetyllactosamine-core (GlcNAcβ1-4Glc) glycans, hexose oligomers,N-glycans and to our surprise β1-3 galactosylglucose-core (Galβ1-3Glc) glycans. Although glycans with a β1-3 galactosylglucose-core were identified as major components in urine, comprising structurally simple isomers of a lactose-core, the core structure has not previously been reported. The major β1-3 galactosylglucose-core glycans were Fucα1-2Galβ1-3(Fucα1-4)Glc, GalNAcα1-3(Fucα1-2)Galβ1-3(Fucα1-4)Glc and Galα1-3(Fucα1-2)Galβ1-3(Fucα1-4)Glc, corresponding to H-, A-, and B-blood group antigens, respectively. Three lactosamine extended β1-3 galactosylglucose-core glycans were also detected as minor components. Elucidating the biosynthesis of β1-3 galactosylglucose will be crucial for understanding thein vivofunction of these glycans.