High levels of oxidative stress globally inhibit gene transcription and histone acetylation
High levels of oxidative stress globally inhibit gene transcription and histone acetylation
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DOI：
10.1089/dna.2006.25.124
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发表时间：
2006-02-01
影响因子：
3.1
通讯作者：
Gaudreau, L
中科院分区：
文献类型：
--
作者：
Berthiaume, M;Boufaied, N;Gaudreau, L
氧化应激已被证明可诱导RNA聚合酶II的泛化，但这一现象与全球转录的直接关系仍然难以捉摸。在这份报告中，我们表明高水平的细胞氧化应激在全球范围内抑制基因转录，这种转录减少只是部分归因于RNA聚合酶II与模型基因启动子结合的减少。重要的是，我们发现这种转录水平的下降与组蛋白H3和H4乙酰化水平的显著下降有关，无论是在整个模型基因中，还是在细胞核中都是如此。我们的结果表明，高水平的氧化应激可以通过至少部分抑制组蛋白乙酰化水平的机制来抑制转录。
Oxidative stress has been shown to induce ubiquitynation of RNA polymerase II, but direct bearing of that phenomenon on global transcription still remains elusive. In this report, we show that high levels of cellular oxidative stress globally inhibit gene transcription, and that this decrease in transcription is only partly attributable to reduced binding of RNA polymerase II to a model gene promoter. Importantly, we show that this decrease in transcription correlates with a significant decrease in histone H3 and H4 acetylation levels both throughout a model gene, and also globally in the nucleus of cells. Our results suggest that high levels of oxidative stress can inhibit transcription by a mechanism, at least in part, that empedes global histone acetylation levels.