BILIARY GLYCOPROTEIN MESSENGER-RNA EXPRESSION IS INCREASED IN PRIMARY LUNG-CANCER, ESPECIALLY IN SQUAMOUS-CELL CARCINOMA
BILIARY GLYCOPROTEIN MESSENGER-RNA EXPRESSION IS INCREASED IN PRIMARY LUNG-CANCER, ESPECIALLY IN SQUAMOUS-CELL CARCINOMA
复制标题
DOI：
10.1165/ajrcmb.11.2.8049082
复制
发表时间：
1994-08-01
影响因子：
6.4
通讯作者：
KIRA, S
中科院分区：
文献类型：
--
作者：
OHWADA, A;TAKAHASHI, H;KIRA, S
胆汁糖蛋白（BGP）是癌胚抗原基因家族的成员，是具有跨膜结构域和胞浆结构域的细胞表面糖蛋白。BGPs由至少四种同种型（BGPa、B、c和d）组成，并且在体外作为Ca 2+依赖性同型细胞间粘附分子起作用。本文对15例原发性肺癌（鳞癌6例，腺癌5例，小细胞癌4例）的原发癌和转移癌组织中BGP基因mRNA的表达进行了研究。并与癌旁正常组织进行比较。北方杂交和原位杂交结果显示，BGP mRNA在原发灶癌组织中表达增高，尤其在鳞癌中，而在癌旁正常组织中未见表达。有趣的是，BGP mRNA的表达明显降低转移灶相比，在6个人的鳞状细胞癌的原发部位。此外，通过逆转录-聚合酶链反应在6例鳞状细胞癌患者中的4例中观察到BGPa亚型的丢失。这些结果表明，BGP表达的减少可能通过减少与周围细胞的粘附相互作用在转移过程中发挥重要作用，特别是在鳞状细胞癌中。
Biliary glycoprotein (BGP), a member of the carcinoembryonic antigen gene family, is a cell surface glycoprotein that has both a transmembrane domain and a cytoplasmic domain. BGPs consist of at least four isoforms (BGPa, b, c, and d) and function in vitro as Ca2+-dependent homotypic intercellular adhesion molecules. The mRNA expression of BGP gene was investigated in specimens of primary and metastatic cancer tissues from 15 patients with primary lung cancer (six squamous cell carcinomas, five adenocarcinomas, and four small cell carcinomas). The specimens were also compared with normal adjacent tissues of the same individuals with squamous cell carcinoma. BGP mRNA expression was increased in carcinomatous tissues of the primary site, especially in squamous cell carcinoma, but was not detected in adjacent normal tissues by Northern blot analysis or in situ hybridization. Interestingly, BGP mRNA expression was apparently decreased in metastatic lesions compared with the primary site in the six individuals with squamous cell carcinoma. Furthermore, a loss of BGPa isoform was observed by reverse transcriptase-polymerase chain reaction in four of six patients with squamous cell carcinoma. These results suggest that the reduction of BGP expression may play an important role in the process of metastasis through decreasing adhesive interactions with surrounding cells, especially in squamous cell carcinoma.