Mega-Dalton biomolecular motion captured from electron microscopy reconstructions
Mega-Dalton biomolecular motion captured from electron microscopy reconstructions
复制标题
DOI：
10.1016/s0022-2836(02)01426-2
复制
发表时间：
2003-02-14
影响因子：
5.6
通讯作者：
Wriggers, W
中科院分区：
文献类型：
--
作者：
Chacón, P;Tama, F;Wriggers, W
弹性模型的振动分析表明，大的生物分子组件的基本运动可以有效地捕获在一个中间尺度，而不需要的原子结构的知识。虽然以前的工作已经建立了一个理论基础，这种分析，我们在这里证明实验电子显微镜图，振动模式确实描述功能相关的运动的大分子机器。细菌RNA聚合酶中的钳夹闭合、核糖体的30 S和50 S亚基的棘轮效应以及伴侣蛋白CCT的动态柔性直接从15-27埃分辨率的单电子显微镜结构中提取。所提出的结果与实验观察到的运动的惊人的一致性表明，细胞中的大尺度机械的运动是令人惊讶的独立于详细的原子相互作用，可以相当合理地描述为弹性体的运动。(C)2003爱思唯尔科技有限公司版权所有。
The vibrational analysis of elastic models suggests that the essential motions of large biomolecular assemblies can be captured efficiently at an intermediate scale without requiring knowledge of the atomic structure. While prior work has established a theoretical foundation for this analysis, we demonstrate here on experimental electron microscopy maps that vibrational modes indeed describe functionally relevant movements of macromolecular machines. The clamp closure in bacterial RNA polymerase, the ratcheting of 30 S and 50 S subunits of the ribosome, and the dynamic flexibility of chaperonin CCT are extracted directly from single electron microscopy structures at 15-27 Angstrom resolution. The striking agreement of the presented results with experimentally observed motions suggests that the motion of the large scale machinery in the cell is surprisingly independent of detailed atomic interactions and can be quite reasonably described as a motion of elastic bodies. (C) 2003 Elsevier Science Ltd. All rights reserved.