Low dose of fine particulate matter (PM2.5) can induce acute oxidative stress, inflammation and pulmonary impairment in healthy mice
Low dose of fine particulate matter (PM2.5) can induce acute oxidative stress, inflammation and pulmonary impairment in healthy mice
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DOI：
10.3109/08958378.2011.566290
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发表时间：
2011-04-01
影响因子：
2.1
通讯作者：
Zin, W. A.
中科院分区：
文献类型：
--
作者：
Riva, D. R.;Magalhaes, C. B.;Zin, W. A.
空气污染与呼吸道疾病引起的发病率和死亡率有关。然而，其中涉及的机制尚未完全阐明。因此，我们测试了一种假设，即单一急性暴露于低剂量细颗粒物(PM2.5)可能会导致肺功能和组织学变化，并解除炎症和氧化应激过程。对圣保罗市城区24 h内采集的PM2.5进行元素和多环芳烃含量分析。4 6只雄性BALB/c小鼠鼻腔内分别滴入L生理盐水30亩(P<0.0 5)或PM2.5，剂量分别为5或15 U/g(P<0.0 5)。24小时后，测定肺力学指标。然后取肺做组织学和生化分析。P15组大鼠肺阻抗明显增加，肺泡塌陷，肺组织炎症、氧化应激和损伤明显增加。P5的值介于CTRL和P15之间：机械阻抗和炎症高于CTRL，但炎症和氧化应激低于P15。总之，急性暴露于低剂量细颗粒物可诱导小鼠肺部炎症、氧化应激，并以剂量依赖的方式恶化肺阻抗和组织学。
Air pollution is associated with morbidity and mortality induced by respiratory diseases. However, the mechanisms therein involved are not yet fully clarified. Thus, we tested the hypothesis that a single acute exposure to low doses of fine particulate matter (PM2.5) may induce functional and histological lung changes and unchain inflammatory and oxidative stress processes. PM2.5 was collected from the urban area of Sao Paulo city during 24 h and underwent analysis for elements and polycyclic aromatic hydrocarbon contents. Forty-six male BALB/c mice received intranasal instillation of 30 mu L of saline (CTRL) or PM2.5 at 5 or 15 mu g in 30 mu L of saline (P5 and P15, respectively). Twenty-four hours later, lung mechanics were determined. Lungs were then prepared for histological and biochemical analysis. P15 group showed significantly increased lung impedance and alveolar collapse, as well as lung tissue inflammation, oxidative stress and damage. P5 presented values between CTRL and P15: higher mechanical impedance and inflammation than CTRL, but lower inflammation and oxidative stress than P15. In conclusion, acute exposure to low doses of fine PM induced lung inflammation, oxidative stress and worsened lung impedance and histology in a dose-dependent pattern in mice.