The Effects of Regular Exercise on Circulating Cardiovascular-related MicroRNAs
The Effects of Regular Exercise on Circulating Cardiovascular-related MicroRNAs
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DOI：
10.1038/s41598-019-43978-x
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发表时间：
2019-05-17
影响因子：
4.6
通讯作者：
Sarzynski, Mark A.
中科院分区：
文献类型：
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作者：
Barber, Jacob L.;Zellers, Kia N.;Sarzynski, Mark A.
本研究的目的是检查定期运动对靶向循环microRNA（miRNAs）丰度的影响。本分析研究了20名以前久坐不动的成年人从遗产家庭研究谁完成了20周的耐力运动训练。通过对人类心血管疾病miRNA阵列（Qiagen，德国）进行RT-qPCR，测量在基线和训练后收集的血清中与心血管疾病相关的53种miRNA的表达。使用基线和训练后表达水平的配对t检验评估定期运动对循环miRNA的影响。使用5%的错误发现率阈值来确定显著性。定期运动导致9种miRNAs的平均血清表达显著降低（miR-486- 5 p、let-7 b-5 p、miR-29 c-3 p、let-7 e-5 p、miR-93- 5 p、miR-7- 5 p、miR-25- 3 p、miR-92 a-3 p和miR-29 b-3 p;倍数变化范围：0.64-83，p =0.0002-0.01）和5种miRNA的平均表达增加（miR-142- 3 p、miR-221- 3 p、miR-126- 3 p、miR-146 a-5 p和miR-27 b-3 p;倍数变化范围：1.41-3.60，p = 0.001-0.006）。富集分析发现，这14种miRNAs靶向与超过345种不同生物学途径相关的基因。这些结果进一步证明了定期运动对循环miRNA谱的影响。
The purpose of the present study was to examine the effects of regular exercise on the abundance of targeted circulating microRNAs (miRNAs). The present analysis examined 20 previously sedentary adults from the HERITAGE Family Study who completed 20 weeks of endurance exercise training. The expression of 53 miRNAs related to cardiovascular disease were measured in serum collected at baseline and post-training by performing RT-qPCR on the Human Cardiovascular Disease miRNA array (Qiagen, Germany). The effect of regular exercise on circulating miRNAs was assessed using paired t-tests of baseline and post-training expression levels. A false discovery rate threshold of 5% was used to determine significance. Regular exercise resulted in significantly decreased mean serum expression of nine miRNAs (miR-486-5p, let-7b-5p, miR-29c-3p, let-7e-5p, miR-93-5p, miR-7-5p, miR-25-3p, miR-92a-3p, and miR-29b-3p; fold change range: 0.64-83, p =0.0002-0.01) and increased mean expression of five miRNAs (miR-142-3p, miR-221-3p, miR-126-3p, miR-146a-5p, and miR-27b-3p; fold change range: 1.41-3.60, p = 0.001-0.006). Enrichment analysis found that these 14 miRNAs target genes related to over 345 different biological pathways. These results provide further evidence of the effects of regular exercise on the circulating miRNA profile.