Human SRMAtlas: A Resource of Targeted Assays to Quantify the Complete Human Proteome.
Human SRMAtlas: A Resource of Targeted Assays to Quantify the Complete Human Proteome.
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人类 SRMAtlas：量化完整人类蛋白质组的靶向测定资源。
DOI：
10.1016/j.cell.2016.06.041
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发表时间：
2016-07-28
期刊：
影响因子：
64.5
通讯作者：
Moritz RL
中科院分区：
文献类型：
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作者：
Kusebauch U;Campbell DS;Deutsch EW;Chu CS;Spicer DA;Brusniak MY;Slagel J;Sun Z;Stevens J;Grimes B;Shteynberg D;Hoopmann MR;Blattmann P;Ratushny AV;Rinner O;Picotti P;Carapito C;Huang CY;Kapousouz M;Lam H;Tran T;Demir E;Aitchison JD;Sander C;Hood L;Aebersold R;Moritz RL
可靠和可重复地测量任何组织或细胞类型中人类蛋白质组的任何蛋白质的能力对于理解系统水平的特性以及生理学和疾病中的特定途径将是变革性的。在这里，我们描述了高度特异性测定的概要的生成和验证，该测定能够通过广泛可及的、灵敏的和稳健的靶向质谱方法选择反应监测SRM来定量20，277种注释的人类蛋白质中的99.7%。该人SRMAtlas提供了决定性的坐标，其决定性地鉴定生物样品中的相应肽。我们报告了166，174种蛋白质型肽的数据，这些肽提供了多种独立的测定方法，用于定量任何人类蛋白质和许多剪接变体、非同义突变和翻译后修饰。这些数据可以在www.srmatlas.org上作为资源免费获得，我们通过检查肝细胞中胆固醇合成抑制和前列腺癌细胞系中多西他赛的网络反应来证明其效用。
The ability to reliably and reproducibly measure any protein of the human proteome in any tissue or cell-type would be transformative for understanding systems-level properties as well as specific pathways in physiology and disease. Here we describe the generation and verification of a compendium of highly specific assays that enable quantification of 99.7% of the 20,277 annotated human proteins by the widely accessible, sensitive and robust targeted mass spectrometric method selected reaction monitoring, SRM. This human SRMAtlas provides definitive coordinates that conclusively identify the respective peptide in biological samples. We report data on 166,174 proteotypic peptides providing multiple, independent assays to quantify any human protein and numerous spliced variants, non-synonymous mutations and post-translational modifications. The data is freely accessible as a resource at www.srmatlas.org, and we demonstrate its utility by examining the network response to inhibition of cholesterol synthesis in liver cells and to docetaxel in prostate cancer lines.