High-Sensitivity Method for Counting Low Concentrations of Particles in Liquids Using Centrifugation and Image Analysis
High-Sensitivity Method for Counting Low Concentrations of Particles in Liquids Using Centrifugation and Image Analysis
复制标题
利用离心和图像分析对液体中低浓度颗粒进行计数的高灵敏度方法
DOI：
10.1143/jjap.39.3641
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Hiraki Okada
中科院分区：
文献类型：
--
作者：
K. Yabusaki;Naoko Saitoh;T. Hirono;H. Matsui;Hiraki Okada
本文提出了一种新的方法来计算悬浮在液体中的低浓度颗粒，并发现这种方法只受泊松统计的限制。该方法被称为图像细胞计数-离心（ICC）法，利用离心将颗粒集中到特殊设计的比色皿底部的狭窄观察区域，然后使用图像分析来测量颗粒数量。我们的方法用于计数悬浮在500 µl液体中的1至64个荧光珠。通过比较计数的珠粒的数量与添加到液体中的珠粒的实际数量，我们证实了ICC方法的计数精度受到泊松计数统计的限制。
A new method was developed for counting low concentrations of particles suspended in a liquid, and found to be limited only by Poisson statistics. The method, called the image cytometry-centrifugation (ICC) method, uses centrifugation to concentrate particles into a narrow observation area at the bottom of a specially designed cuvette, and then uses image analysis to measure the number of particles. Our method was used to count from 1 to 64 fluorescent beads suspended in 500 µl of liquid. By comparing the number of counted beads with the actual number of beads added to the liquid, we confirmed that the counting accuracy of the ICC method is limited by Poisson counting statistics.