SURFACE MULTI-FUNCTIONALIZATION OF POLY(LACTIC ACID) NANOPARTICLES AND C6 GLIOMA CELL TARGETING in vivo
SURFACE MULTI-FUNCTIONALIZATION OF POLY(LACTIC ACID) NANOPARTICLES AND C6 GLIOMA CELL TARGETING in vivo
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DOI：
10.1142/s0256767909003868
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发表时间：
2009-03
影响因子：
4.3
通讯作者：
Xubo Yuan;C. Kang;Yunhui Zhao;Ming-qi Gu;P. Pu;Neng-jiang Tian;J. Sheng
中科院分区：
文献类型：
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作者：
Xubo Yuan;C. Kang;Yunhui Zhao;Ming-qi Gu;P. Pu;Neng-jiang Tian;J. Sheng
采用疏水胆固醇修饰葡聚糖聚醛和聚乳酸的DMSO溶液对水进行透析，制备了含醛基聚乳酸多糖包被纳米粒子。纳米颗粒的平均直径约为160 nm，粒径分布基本均匀。通过席夫碱反应将纳米颗粒与CD71和EGFR抗体同时功能化，然后用99mTc进行放射性标记。将放射性标记的纳米颗粒经左颈总动脉灌注至荷瘤大鼠体内，用闪烁计数器测定其肝、脾、肾、脑的放射性水平。结果表明，由于RES系统对纳米颗粒的摄取，只有不到2%的纳米颗粒被大脑吸收。然而，转铁蛋白抗体在纳米颗粒上的偶联促进了纳米颗粒穿过血脑屏障，更特别的是，与单功能化和天然纳米颗粒相比，多功能化增强了纳米颗粒在体内的肿瘤积累。
Polysaccharide coated PLA nanoparticles bearing aldehyde groups were prepared by dialysis of DMSO solution of cholesterol hydrophobic-modified dextran polyaldehyde and PLA against water. The average diameter of the nanoparticles was about 160 nm, and the size distribution was nearly homogenous. The nanoparticles were functionalized simultaneously with CD71 and EGFR antibody through the Schiff's base reaction, and then radiolabeled with 99mTc. After perfused the radiolabeled nanoparticles into tumor-bearing rats through left common carotid artery, the radioactivity in liver, spleen, kidney and brain was measured by scintillation counter. The results showed that less than 2% of nanoparticles were uptaken by the brain due to the uptake of the nanoparticles by the RES system. However, the coupling of transferrin antibody on the nanoparticles facilitated the penetration of nanoparticles across the blood brain barrier, and more specially, compared with monofuctionalized and native nanoparticles, the multifunctionalization enhanced the tumor accumulation of the nanoparticles in vivo.