Measurement of cerebral vascular extraction fractions in the rat using intracarotid injection techniques
Measurement of cerebral vascular extraction fractions in the rat using intracarotid injection techniques
复制标题
使用颈动脉注射技术测量大鼠脑血管提取分数
DOI：
10.1016/0006-8993(81)90560-6
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
K. B. Larson
中科院分区：
文献类型：
--
作者：
H. Clark;K. HartmanBoyd;E. RaichleMarcus;H. PreskornSheldon;K. B. Larson
描述了一种小体积（5μl）颈总动脉注射方法，用于定量大鼠中扩散限制示踪分子的脑血管提取分数（Et）。该方法是奥尔登多夫提出的扩散技术的改进，广泛用于血脑屏障现象的研究。虽然 Oldendorf 技术已被证明对于估计物质的相对渗透性很有价值，但它在测量生理或药理学改变的渗透性或血流的 Etunder 条件方面受到限制。本文描述的方法 - 使用小体积 (5 μl) 颈总动脉注射液、可自由扩散的参考示踪剂、[14C]丁醇和 5 秒循环时间 - 可以测量反映血流变化和渗透性微小差异的 Et。改进的方法对于研究廉价动物模型中脑血管通透性和流量的调节具有重要意义。
A small volume (5 μl) common carotid arterial injection method is described for the quantitation of cerebral vascular extraction fractions (Et) of diffusion limited tracer molecules in the rat. The method is a modification of a technique diffusion duced by Oldendorf and widely used for the study of blood-brain barrier phenomena. While the Oldendorf technique has proven valuable for estimating the relative permeabilities of substances, it is limited in measuring Etunder conditions of physiologically or pharmacologically altered permeability or blood flow. The method described in this paper — using a small volume (5 μl) common carotid injection, a freely diffusible reference tracer, [14C]butanol, and a 5 sec circulation time — allows for measurements of Etthat reflect changes in blood flow and small differences in permeability. The modified method is important for the study of the regulation of cerebral vascular permeability and flow in an inexpensive animal model.