Dynamic sex chromosome expression in Drosophila male germ cells.
Dynamic sex chromosome expression in Drosophila male germ cells.
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DOI：
10.1038/s41467-021-20897-y
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发表时间：
2021-02-09
影响因子：
16.6
通讯作者：
Oliver B
中科院分区：
文献类型：
--
作者：
Mahadevaraju S;Fear JM;Akeju M;Galletta BJ;Pinheiro MMLS;Avelino CC;Cabral-de-Mello DC;Conlon K;Dell'Orso S;Demere Z;Mansuria K;Mendonça CA;Palacios-Gimenez OM;Ross E;Savery M;Yu K;Smith HE;Sartorelli V;Yang H;Rusan NM;Vibranovski MD;Matunis E;Oliver B
鉴于它们的拷贝数差异和独特的遗传模式，进化的基因内容和性染色体的表达是不寻常的。在许多生物体中，精母细胞中的 X 和 Y 染色体失活，可能是作为一种防御机制，防止插入不配对的染色质。除了当前的性染色体外，果蝇还有一个小的基因贫乏的 X 染色体遗迹（第四条），它重新获得了常染色体状态。在这里，我们在果蝇幼虫上使用单细胞 RNA 测序，基于测量我们确定的睾丸中的所有基因或一组广泛表达的基因，证明单个 X 染色体和一对第四染色体在初级精母细胞中特异性失活。相比之下，单个 Y 染色体上的基因在初级精母细胞中变得最活跃。 X 转录物水平降低是由于丝氨酸 2 和 5 磷酸化未能激活 RNA 聚合酶-II。性染色体基因含量和表达异常。在这里，作者在果蝇幼虫上使用单细胞 RNA 测序来证明，单条 X 染色体和一对第四染色体在初级精母细胞中特异性失活，而单条 Y 染色体上的基因在初级精母细胞中变得最大程度活跃。
Given their copy number differences and unique modes of inheritance, the evolved gene content and expression of sex chromosomes is unusual. In many organisms the X and Y chromosomes are inactivated in spermatocytes, possibly as a defense mechanism against insertions into unpaired chromatin. In addition to current sex chromosomes, Drosophila has a small gene-poor X-chromosome relic (4th) that re-acquired autosomal status. Here we use single cell RNA-Seq on fly larvae to demonstrate that the single X and pair of 4th chromosomes are specifically inactivated in primary spermatocytes, based on measuring all genes or a set of broadly expressed genes in testis we identified. In contrast, genes on the single Y chromosome become maximally active in primary spermatocytes. Reduced X transcript levels are due to failed activation of RNA-Polymerase-II by phosphorylation of Serine 2 and 5. Sex chromosome gene content and expression is unusual. Here the authors use single cell RNA-Seq on Drosophila larvae to demonstrate that the single X and pair of 4th chromosomes are specifically inactivated in primary spermatocytes, while genes on the single Y chromosome become maximally active in primary spermatocytes.
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影响因子：
9.8
作者：
Hense, Winfried;Baines, John F.;Parsch, John
通讯作者：
Parsch, John
影响因子：
--
作者：
Gupta V;Parisi M;Sturgill D;Nuttall R;Doctolero M;Dudko OK;Malley JD;Eastman PS;Oliver B
通讯作者：
Oliver B
影响因子：
2.4
作者：
Leser K;Awe S;Barckmann B;Renkawitz-Pohl R;Rathke C
通讯作者：
Rathke C
影响因子：
3.7
作者：
Bakken, Trygve E.;Hodge, Rebecca D.;Tasic, Bosiljka
通讯作者：
Tasic, Bosiljka
DOI：
10.1073/pnas.231484998
发表时间：
2001-11-06
影响因子：
11.1
作者：
Carvalho, AB;Dobo, BA;Clark, AG
通讯作者：
Clark, AG