miR-370 is stage-specifically expressed during mouse embryonic development and regulates Dnmt3a
miR-370 is stage-specifically expressed during mouse embryonic development and regulates Dnmt3a
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miR-370 在小鼠胚胎发育过程中阶段特异性表达并调节 Dnmt3a
DOI：
10.1016/j.febslet.2013.01.070
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发表时间：
2013-03-18
期刊：
影响因子：
3.5
通讯作者：
Wu Qiong
中科院分区：
文献类型：
--
作者：
Liu Qi;He Hongjuan;Wu Qiong
MicroRNAs （miRNAs）是参与多种生物过程的小非编码rna。本文对小鼠胚胎发育过程中miR-370的时空表达模式进行了表征，发现其具有阶段特异性和组织特异性表达。此外，通过荧光素酶报告基因检测和western blot分析，DNA甲基转移酶3A （Dnmt3a）被确定为miR-370的直接调控靶点。总之，我们的研究结果表明，miR-370可能在小鼠发育过程中通过介导Dnmt3a的表达，在多种器官，特别是脑和肾上腺的形态发生中发挥重要作用。(C) 2013年欧洲生化学会联合会。Elsevier b.v.版权所有。
MicroRNAs (miRNAs) are small non-coding RNAs that participate in a large variety of biological processes. In this paper, the spatiotemporal expression pattern of miR-370 was characterized during mouse embryonic development, and was found to be stage-and tissue-specifically expressed. In addition, through luciferase reporter assays and western blot analyses, DNA methyltransferase 3A (Dnmt3a) was identified as a directly regulated target of miR-370. Altogether, our results indicate that miR-370 may play important roles in the morphogenesis of diverse organs, especially brain and adrenal glands, by mediating Dnmt3a expression during mouse development. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.