Discrepancy between Cardiac Troponin Assays Due to Endogenous Antibodies
Discrepancy between Cardiac Troponin Assays Due to Endogenous Antibodies
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DOI：
10.1093/clinchem/hvz032
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发表时间：
2020-03-01
影响因子：
9.3
通讯作者：
Kyle, Campbell
中科院分区：
文献类型：
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作者：
Lam, Leo;Aspin, Lisa;Kyle, Campbell
背景：尽管针对心肌肌钙蛋白（cTn） I的自身抗体在实验分析中有很好的分析作用，但没有研究系统地检查它们对临床使用的cTn分析的影响。我们确定了内源性抗体对5种不同的cTnI检测和一种cTnT检测的影响。方法：cTn采用西门子hs-cTnI Centaur、西门子hs-cTnI Vista、雅培hs-cTnI Architect、贝克曼hs-cTnI Access、贝克曼cTnI Access和罗氏hs-cTnT Elecsys 6种方法测量。在用蛋白a孵育去除免疫球蛋白后，在5项检测中重复测量（除Siemens hs-cTnI Vista外）。
BACKGROUND: Despite well-described analytical effects of autoantibodies against cardiac troponin (cTn) I on experimental assays, no study has systematically examined their impact on cTn assays in clinical use. We determined the effects of endogenous antibodies on 5 different cTnI assays and a cTnT assay.METHODS: cTn was measured by 6 methods: Siemens hs-cTnI Centaur, Siemens hs-cTnI Vista, Abbott hs-cTnI Architect, Beckman hs-cTnI Access, Beckman cTnI Access, and Roche hs-cTnT Elecsys. Measurements were repeated on 5 assays (all except Siemens hs-cTnI Vista) following immunoglobulin depletion by incubation with protein A. Low recovery of cTnI (