Distribution and time course of hair cell regeneration in the pigeon utricle
Distribution and time course of hair cell regeneration in the pigeon utricle
复制标题
DOI：
10.1016/s0378-5955(99)00046-5
复制
发表时间：
1999-07-01
期刊：
影响因子：
2.8
通讯作者：
Dickman, JD
中科院分区：
文献类型：
--
作者：
Dye, BJ;Frank, TC;Dickman, JD
氨基糖苷类抗生素耳毒性损伤后的前庭和耳蜗再生已被充分记录，特别是在鸟类中。在本研究中，耳内应用2毫克链霉素糊剂，以实现完整的前庭毛细胞破坏鸽子（鸽），同时保留再生能力。扫描电子显微镜被用来量化毛细胞密度纵向再生过程中在三个不同的椭圆囊黄斑的位置，包括条纹，中央和周边地区。耳内用链霉素治疗后4天，椭圆囊上皮没有静纤毛（表明毛细胞丢失）。2周时，静纤毛开始随机出现，大多数是不成熟的形式。然而，当目前大多数动纤毛两极化发展的条纹。最初，再生发生更迅速地在中央和周边地区的胞果相比，条纹。随着再生从2到12周进行，在纹状体区域的毛细胞密度等于在中央和周边区域的密度。在24周时，中央和外周区域的毛细胞密度等于正常值，但是纹状体区域的毛细胞密度略高于正常动物。(C)1999 Elsevier Science B.V.保留所有权利。
Vestibular and cochlear regeneration following ototoxic insult from aminoglycoside antibiotics has been well documented, particularly in birds. In the present study, intraotic application of a 2 mg streptomycin paste was used to achieve complete vestibular hair cell destruction in pigeons (Columba livia) while preserving regenerative ability. Scanning electron microscopy was used to quantify hair cell density longitudinally during regeneration in three different utricular macula locations, including the striola, central and peripheral regions. The utricular epithelium was void of stereocilia (indicating hair cell loss) at 4 days after intraotic treatment with streptomycin. At 2 weeks the stereocilia began to appear randomly and mostly in an immature form. However, when present most kinocilia were polarized toward the developing striola. Initially, regeneration occurred more rapidly in the central and peripheral regions of the utricle as compared to the striola. As regeneration proceeded from 2 to 12 weeks, hair cell density in the striola region equaled the density noted in the central and peripheral regions. At 24 weeks, hair cell density of the central and peripheral regions was equal to normal values, however the striola region had a slightly greater hair cell density than that observed for normal animals. (C) 1999 Elsevier Science B.V. All rights reserved.