Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals
Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals
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DOI：
10.1016/j.immuni.2020.04.023
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发表时间：
2020-06-16
期刊：
影响因子：
32.4
通讯作者：
Dong, Chen
中科院分区：
文献类型：
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作者：
Ni, Ling;Ye, Fang;Dong, Chen
世界卫生组织宣布SARS-CoV-2病毒爆发为全球性大流行。然而，人们对SARS-CoV-2感染后的免疫反应，特别是适应性免疫反应的认识非常有限。在这里，我们收集了新冠肺炎患者的血液，这些患者最近已经成为无病毒，因此出院，并检测了8名新出院患者的SARS-CoV-2特异性体液和细胞免疫。出院后2周对另一组6例患者的随访分析也显示免疫球蛋白G（IgG）抗体滴度高。在所有14例受试者中，13例在假型进入试验中显示血清中和活性。值得注意的是，中和抗体滴度与病毒特异性T细胞的数量之间存在强相关性。我们的工作为进一步分析SARS-CoV-2的保护性免疫，了解COVID-19的发病机制，特别是重症病例的发病机制提供了基础。它也对开发有效的SARS-CoV-2感染疫苗具有意义。
The World Health Organization has declared SARS-CoV-2 virus outbreak a worldwide pandemic. However, there is very limited understanding on the immune responses, especially adaptive immune responses to SARS-CoV-2 infection. Here, we collected blood from COVID-19 patients who have recently become virus-free, and therefore were discharged, and detected SARS-CoV-2-specific humoral and cellular immunity in eight newly discharged patients. Follow-up analysis on another cohort of six patients 2 weeks post discharge also revealed high titers of immunoglobulin G (IgG) antibodies. In all 14 patients tested, 13 displayed serum-neutralizing activities in a pseudotype entry assay. Notably, there was a strong correlation between neutralization antibody titers and the numbers of virus-specific T cells. Our work provides a basis for further analysis of protective immunity to SARS-CoV-2, and understanding the pathogenesis of COVID-19, especially in the severe cases. It also has implications in developing an effective vaccine to SARS-CoV-2 infection.