DIRECT OBSERVATION OF MOTION OF SINGLE F-ACTIN FILAMENTS IN THE PRESENCE OF MYOSIN
DIRECT OBSERVATION OF MOTION OF SINGLE F-ACTIN FILAMENTS IN THE PRESENCE OF MYOSIN
复制标题
DOI：
10.1038/307058a0
复制
发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
OOSAWA, F
中科院分区：
文献类型：
--
作者：
YANAGIDA, T;NAKASE, M;OOSAWA, F
肌动蛋白存在于几乎所有类型的非肌肉细胞中，被认为在细胞运动中起重要作用。只有当可靠的体外系统可用于研究细胞肌动蛋白和肌球蛋白的相互作用时，才有可能正确理解这种作用。在几个系统1 -4上已经有了一个开始，最近的是Sheetz和Spudich，他们证明了在通过解剖a Nitellacell暴露的叶绿体阵列上，HMM包被的珠沿着F-肌动蛋白电缆的单向运动5。作为一种替代方法，我们在这里报告的直接观察荧光显微镜的运动的单个F-肌动蛋白丝相互作用的可溶性肌球蛋白片段通电Mg 2 +-ATP。
Actin is found in almost all kinds of non-muscle cells where it is thought to have an important role in cell motility. A proper understanding of that role will only be possible when reliablein vitrosystems are available for investigating the interaction of cellular actin and myosin. A start has been made on several systems1–4, most recently by Sheetz and Spudich who demonstrated unidirectional movement of HMM-coated beads along F-actin cables on arrays of chloroplasts exposed by dissection of aNitellacell5. As an alternative approach, we report here the direct observation by fluorescence microscopy of the movements of single F-actin filaments interacting with soluble myosin fragments energized by Mg2+-ATP.