Regional expression of the type 3 iodothyronine deiodinase messenger ribonucleic acid in the rat central nervous system and its regulation by thyroid hormone.
Regional expression of the type 3 iodothyronine deiodinase messenger ribonucleic acid in the rat central nervous system and its regulation by thyroid hormone.
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DOI：
10.1210/endo.140.2.6486
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发表时间：
1999-02
期刊：
影响因子：
4.8
通讯作者：
H. Tu;G. Légrádi;T. Bartha;D. Salvatore;R. Lechan;P. Larsen
中科院分区：
文献类型：
--
作者：
H. Tu;G. Légrádi;T. Bartha;D. Salvatore;R. Lechan;P. Larsen
3型碘甲状腺原氨酸脱碘酶（D3）是一种使甲状腺激素失活的硒酶。它是中枢神经系统T3稳态所必需的。D3活性已在大脑的许多区域被确定，并与甲状腺状态相似，但其受调节的水平及其特定的细胞位置尚不清楚。我们利用原位杂交组织化学技术评估了甲状腺状态对中枢神经系统D3基因表达的影响。D3信使RNA （mRNA）在海马锥体神经元、齿状核颗粒细胞和大脑皮层II-VI层中均有高表达。在每个区域，D3 mRNA丰度与甲状腺状态相关。Northern分析鉴定了四种不同的D3转录本，有区域特异性加工的证据，从甲状腺功能正常状态到甲状腺功能亢进状态，D3 mRNA增加了4- 50倍。在甲状腺功能减退的脑组织中未检测到D3 mRNA。在中枢神经系统中，D3基因对T3有高度的反应，其在海马和大脑皮层的局灶定位表明T3在记忆和认知功能中的稳态起重要作用。
Type 3 iodothyronine deiodinase (D3) is a selenoenzyme that inactivates thyroid hormone. It is necessary for T3 homeostasis in the central nervous system. D3 activity has been identified in many regions of the brain and parallels thyroid status, but the level at which it is regulated and its specific cellular locations are not known. We evaluated the effect of thyroid status on the expression of the D3 gene within the central nervous system using in situ hybridization histochemistry. D3 messenger RNA (mRNA) was identified throughout, but with high focal expression in the hippocampal pyramidal neurons, granule cells of the dentate nucleus, and layers II-VI of the cerebral cortex. In every region, D3 mRNA abundance was correlated with thyroid status. Four different D3 transcripts were identified by Northern analyses, with evidence for region-specific processing, and D3 mRNA increased 4- to 50-fold from the euthyroid to the hyperthyroid state. D3 mRNA was not detectable in hypothyroid brain. In the central nervous system, the D3 gene is highly T3 responsive, and its focal localization within the hippocampus and cerebral cortex suggests an important role for T3 homeostasis in memory and cognitive functions.