The mouse SLIT family: Secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance
The mouse SLIT family: Secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance
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DOI：
10.1006/dbio.1999.9371
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发表时间：
1999-08-15
影响因子：
2.7
通讯作者：
Ornitz, DM
中科院分区：
文献类型：
--
作者：
Yuan, WL;Zhou, LJ;Ornitz, DM
Slit基因编码果蝇胚胎神经发育所必需的分泌分子。在这里，我们报告的三个狭缝同源物在小鼠中的识别。我们证明，小鼠SLIT 1蛋白可以结合ROBO 1，一个跨膜受体参与轴突导向。这两个整体安装和部分原位杂交研究揭示了独特的和互补的模式的表达的三个小鼠Slit基因和Robo 1，无论是在中枢神经系统和其他发育中的组织。Slit和Robo 1的互补表达模式及其体外相互作用表明了配体-受体关系。所有三个Slit基因在底板中的表达表明，它们可能与果蝇同源物在中线神经发育和轴突引导中具有相同的功能特性。Slit和Robo 1在体节的不同亚部中的互补表达表明它们可能在轴突寻路和神经嵴细胞迁移中起作用。肢体和其他器官中的独特表达模式表明Slit基因家族的其他潜在功能。(C)北京：科学出版社.
The Slit gene encodes a secreted molecule essential for neural development in Drosophila embryos. Here we report the identification of three Slit homologues in the mouse. We demonstrate that the mouse SLIT1 protein can bind ROBO1, a transmembrane receptor implicated in axon guidance. Both whole-mount and section in situ hybridization studies reveal unique and complementary patterns of expression of the three mouse Slit genes and of Robo1, both within the central nervous system and in other developing tissues. The complementary expression patterns of Slit and Robo1 and their in vitro interaction suggest a ligand-receptor relationship. The expression of all three Slit genes in the floor plate suggests that they are likely to share the same functional properties with their Drosophila homologue in midline neural development and axon guidance. The complementary expression of Slit and Robo1 in different subdivisions of the somites suggests their possible function in axon pathfinding and neural crest cell migration. The unique expression pattern in Limb and other organs indicates additional potential functions of the Slit gene family. (C) 1999 Academic Press.