1064-nm-resonant gold nanorods for photoacoustic theranostics within permissible exposure limits
1064-nm-resonant gold nanorods for photoacoustic theranostics within permissible exposure limits
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DOI：
10.1002/jbio.201900082
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发表时间：
2019-10-01
影响因子：
2.8
通讯作者：
Pini, Roberto
中科院分区：
文献类型：
--
作者：
Cavigli, Lucia;Centi, Sonia;Pini, Roberto
基于光机械效应的治疗和诊断方法在肿瘤学、心脏病学和血管外科领域以及光声成像或显微外科等应用中引起了人们的广泛关注。其基本机制是短光脉冲与内源性染料或靶向造影剂相互作用产生超声波或空化。在后者中，金纳米棒是杰出的候选者，但其用途主要被报道用于光声成像和光热治疗。相反，人们对它们作为光机械操作精密工具的价值知之甚少，例如通过微泡的膨胀和破裂以高空间分辨率造成局部损伤。在这里，我们讨论了金纳米棒作为光声治疗诊断造影剂的可行性，该金纳米棒表现出表面等离子体共振分布在约 900 至 1100 nm 以上之间。在测试了它们的细胞毒性和细胞摄取后，我们讨论了它们的光稳定性以及用于介导空化和目标细胞的光机械破坏的用途。我们发现，选择峰值在 1064 nm 附近的等离子体带是增强该方法转化潜力的关键。相对于800nm的标准替代品，1064nm对于光学曝光的相关规定限制较少，光子技术也更加成熟。
Therapeutic and diagnostic methods based on photomechanical effects are attracting much current attention in contexts as oncology, cardiology and vascular surgery, for such applications as photoacoustic imaging or microsurgery. Their underlying mechanism is the generation of ultrasound or cavitation from the interaction of short optical pulses with endogenous dyes or targeted contrast agents. Among the latter, gold nanorods are outstanding candidates, but their use has mainly been reported for photoacoustic imaging and photothermal treatments. Conversely, much less is still known about their value as a precision tool for photomechanical manipulations, such as to impart local damage with high spatial resolution through the expansion and collapse of microbubbles. Here, we address the feasibility of gold nanorods exhibiting a distribution of surface plasmon resonances between about 900 to above 1100 nm as a contrast agent for photoacoustic theranostics. After testing their cytotoxicity and cellular uptake, we discuss their photostability and use to mediate cavitation and the photomechanical destruction of targeted cells. We find that the choice of a plasmonic band peaking around 1064 nm is key to enhance the translational potential of this approach. With respect to the standard alternative of 800 nm, at 1064 nm, relevant regulations on optical exposure are less restrictive and the photonic technology is more mature.