Host Range and Complete Genome Sequence of Cucurbit chlorotic yellows virus, a New Member of the Genus Crinivirus
Host Range and Complete Genome Sequence of Cucurbit chlorotic yellows virus, a New Member of the Genus Crinivirus
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DOI：
10.1094/phyto-100-6-0560
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发表时间：
2010-06-01
期刊：
影响因子：
3.2
通讯作者：
Sugiyama, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Okuda, Mitsuru;Okazaki, Shinichiro;Sugiyama, Mitsuhiro
黄瓜黄化病毒（Cucurbit Morale yellows virus，CCYV）是引起黄瓜（Cucumis sativus）和甜瓜（Commis梅洛）黄化的主要病毒，由烟粉虱（Bemisia tabaci）生物型B和Q粉虱传播。为了表征CCYV的宿主范围，使用粉虱载体接种21个葫芦科和12个其他植物物种。所有测试的黄瓜属物种。除了船长吗和黄瓜（Cucumis zeyheri）均被CCYV系统感染，但不同种间感染率不同。西瓜、西葫芦和丝瓜对CCYV较敏感，但感染率较低，症状不明显。除了葫芦科植物。甜菜苋色藜、藜、菠菜、莴苣、曼陀罗和烟草也被CCYV系统感染。对完整的RNA 1和RNA 2进行逆转录、克隆和测序。CCYV RNA 1全长8，607个核苷酸（nt），含有4个开放阅读框（ORF）。第一个ORF跨越甲基转移酶和RNA解旋酶结构域，随后是RNA依赖性RNA聚合酶结构域，推测由+1核糖体移码翻译。CCYV RNA 2全长8,041 nt，包含8个开放阅读框（ORF），包括Closteroviridae的标志基因阵列。系统进化分析表明，CCYV与莴苣退绿病毒的遗传关系较近。菜豆黄色障碍病毒和葫芦黄色发育障碍病毒。这些病毒的代表性蛋白质的氨基酸序列相似性表明，CCYV应被归类为一个独特的海百合病毒种。
Cucurbit Morale yellows virus (CCYV) causes chlorotic yellows on cucumber (Cucumis sativus) and melon (Commis melo) and is transmitted by Bemisia tabaci biotype B and Q whiteflies. To characterize the host range of CCYV, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. All tested Cucumis spp. except Commis argotic? and Cucumis zeyheri were systemically infected with CCYV, although infection rates varied among species. Citrullus lanatus, Cucurbita pepo, and Luffa cylindrica were susceptible to CCYV; however, the infection rates were low and symptoms were unclear. In addition to the cucurbitaceous plants. Beta vulgaris. Chenopodium amaranticolor, Chenopodium quinoa, Spinacia oleracea, Lactuca sativa, Datum stramonium, and Nicotiana benthamiana were also systemically infected by CCYV. Complete RNA1 and RNA2 were reverse-transcribed, cloned, and sequenced. CCYV RNA1 was found to be 8,607 nucleotides (nt) long and contained four open reading frames (ORFs). The first ORF spanned methyltransferase and RNA helicase domains followed by an RNA-dependent RNA polymerase domain, presumably translated by a +1 ribosomal frameshift. CCYV RNA2 was found to be 8,041 nt long and contained eight ORFs, including the hallmark gene array of the family Closteroviridae. Phylogenetic analysis demonstrated that CCYV was genetically close to Lettuce chlorosis virus. Bean yellow disorder virus, and Cucurbit yellow stunting disorder virus. Amino acid sequence similarities of representative proteins with these viruses indicated that CCYV should be classified as a distinct crinivirus species.