Agrimol B present in Agrimonia pilosa Ledeb impedes cell cycle progression of cancer cells through G0 state arrest
Agrimol B present in Agrimonia pilosa Ledeb impedes cell cycle progression of cancer cells through G0 state arrest
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龙牙草中的 Agrimol B 通过 G0 状态阻滞阻碍癌细胞的细胞周期进展
DOI：
10.1016/j.biopha.2021.111795
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发表时间：
2021-06-04
影响因子：
7.5
通讯作者：
Dong, Qihan
中科院分区：
文献类型：
--
作者：
Hnit, Su Su Thae;Ding, Rongzhen;Dong, Qihan
癌症复发是一个重大挑战。在细胞水平上，复发是由于处于G（0）期的休眠癌细胞的再活化而发生的。本研究的目的是从中药新配方--收敛方中筛选出能将前列腺癌和肺癌细胞阻滞在G（0）期的化合物，该配方由白芍、仙鹤草、乌梅、浙贝、甘草、灵芝（Ganoderma Luccium Karst）和膜荚黄芪（Astragalus membranaceus（菲施.）邦吉通过对分别用Hoechst 33342和Pyronin Y染色的癌细胞进行DNA和RNA的流式细胞术分析，通过使前列腺癌和肺癌细胞在G（0）期四舍五入来阻止细胞周期进程。发现该处方的抗癌功效可归因于龙芽草。进一步的研究表明，仙鹤草中提取的一种多酚成分-仙鹤草酚B（agrimol B）对该草药的活性有贡献。通过免疫印迹和免疫荧光分析，发现仙鹤草酚B对癌细胞的作用可能是通过其对c-MYC、SKP 2和p27的影响。口服给予龙芽草B或仙鹤草酚B可减少动物前列腺癌细胞异种移植物的生长。总之，Agriol B可以富集G（0）状态的前列腺癌和肺癌细胞，并影响控制G（0）状态的关键调节因子。
Cancer recurrence poses a significant challenge. At the cellular level, recurrence takes place as a result of reactivation of dormant cancer cells residing at G(0) phase. The aim of the study was to identify compounds that can trap prostate and lung cancer cells in G(0) phase from a new Chinese herb recipe, Astringent recipe, consisting of Radix Paeoniae Alba, Agrimonia pilosa Ledeb, Fructus Mume, Fritillaria thunbergii Miq., Ganoderma Lucidum Karst, and Astragalus membranaceus (Fisch.) Bunge. Astringent recipe impeded cell cycle progression in prostate and lung cancer cells by rounding them up at G(0) phase by flow cytometric analysis of cancer cells stained with Hoechst 33342 and Pyronin Y, respectively, for DNA and RNA. The anti-cancer efficacy of the recipe was found to be attributable to Agrimonia pilosa Ledeb. Further study established that agrimol B, a polyphenol derived from Agrimonia pilosa Ledeb, contributed to the activity of the herb. The action of agrimol B on the cancer cells was likely derived from its effect on c-MYC, SKP2 and p27 by immunoblotting and immunofluorescence. Oral administration of Agrimonia pilosa Ledeb or agrimol B reduced growth of prostate cancer cell xenograft in animal. In conclusion, Agrimol B can enrich for prostate and lung cancer cells in G(0) state and influence key regulators that govern G(0) status.