Chitosan-hyaluronate hybrid gel intraarticular injection delays osteoarthritis progression and reduces pain in a rat meniscectomy model as compared to saline and hyaluronate treatment.
Chitosan-hyaluronate hybrid gel intraarticular injection delays osteoarthritis progression and reduces pain in a rat meniscectomy model as compared to saline and hyaluronate treatment.
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DOI：
10.1155/2012/979152
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发表时间：
2012
影响因子：
1.3
通讯作者：
Robinson D
中科院分区：
文献类型：
--
作者：
Patchornik S;Ram E;Ben Shalom N;Nevo Z;Robinson D
壳聚糖-透明质酸杂化凝胶（CHHG）是一种自形成的温敏性水凝胶。本研究旨在观察中药复方对大鼠骨关节炎的治疗作用。方法.本研究纳入13只大鼠。在所有大鼠中，使用林顿不电容测试仪评估承重。所有大鼠均行双侧内侧部分椎间盘切除术。四只大鼠在对照膝关节中接受生理盐水注射，在实验膝关节中接受200微升CHHG注射。五只大鼠接受了高分子量透明质酸注射到对照膝关节和200微升CHHG注射在实验膝关节。四只大鼠接受相同的外科手术，允许恢复七天，然后注射CHHG和透明质酸盐。对动物进行了6周的随访。在注射治疗物质两周后，使用林顿不电容计评估每个膝关节上的负重量。结果诱导骨关节炎后两周，CHHG治疗的膝关节疼痛比对照治疗的膝关节疼痛轻，如使用Lintron不电容计测定的。6周后，CHHG治疗膝关节的组织学外观上级对照组。这表现为股骨内侧髁上保留的软骨较厚，以及CHHG治疗膝关节中的囊肿形成较少。讨论CHHG似乎延迟骨关节炎的进展，并减轻大鼠膝关节骨关节炎模型中的疼痛。这些结果支持其他已发表的结果，表明壳聚糖对人和兔骨关节炎有改善作用。
Chitosan-Hyaluronate hybrid gel (CHHG) is a self-forming thermo-responsive hydrogel. The current study was undertaken in order to assess the effect of CHHG on rat's surgically induced osteoarthritis. Methods. Thirteen rats were included in the study. In all rats weight-bearing was assessed using a Linton Incapacitance tester. All rats underwent bilateral medial partial meniscectomy. Four rats received a saline injection in the control knee and a 200-microliter injection of CHHG in the experimental knee. Five rats received a high-molecular weight hyaluronate injection to the control knee and a 200-microliter injection of CHHG in the experimental knee. Four rats underwent the same surgical procedure, allowed to recuperate for seven days and then CHHG and hyaluronate were injected. The animals were followed for 6 weeks. Two weeks after injection of a therapeutic substance the amount of weight-bearing on each knee was evaluated using a Linton Incapacitance meter. Results. Two weeks after induction of osteoarthritis there is less pain in the CHHG-treated knee than in the control-treated knee, as determined using a Lintron Incapacitance meter. After six-weeks the histological appearance of the CHHG-treated knee was superior to that of the controls. This is indicated by thicker cartilage remaining on the medial femoral condyle as well as less cyst formation in the CHHG-treated knee. Discussion. CHHG appears to delay progression of osteoarthritis and lessen pain in a rat surgically-induced knee osteoarthritis model. These results support other published results, indicating that there is an ameliorative effect of chitosan on human and rabbit osteoarthritis.