Shugoshin promotes sister kinetochore biorientation in Saccharomyces cerevisiae
Shugoshin promotes sister kinetochore biorientation in Saccharomyces cerevisiae
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DOI：
10.1091/mbc.e07-06-0584
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
Marston, Adele L.
中科院分区：
文献类型：
--
作者：
Kiburz, Brendan M.;Amon, Angelika;Marston, Adele L.
在有丝分裂和减数分裂细胞分裂过程中，染色体分离必须准确地进行。Sgo1至少以两种方式在确保忠实的染色体分离中起关键作用。在减数分裂过程中，这种蛋白质调节着将姐妹染色单体连接在一起的内聚蛋白从染色体中移除。在有丝分裂过程中，Sgo1被用于感知姊妹着丝点没有附着在来自相反极点的微管上而导致的张力缺失。在这里，我们描述了同源染色体和姐妹染色单体分离对Sgo1的不同要求。Sgo1在减数分裂i中同源染色体的分离中只起很小的作用，相反，Sgo1对姐妹着丝点的双向偏向很重要。我们认为Sgo1在姐妹动着点上起作用以促进它们的双性取向。
Chromosome segregation must be executed accurately during both mitotic and meiotic cell divisions. Sgo1 plays a key role in ensuring faithful chromosome segregation in at least two ways. During meiosis this protein regulates the removal of cohesins, the proteins that hold sister chromatids together, from chromosomes. During mitosis, Sgo1 is required for sensing the absence of tension caused by sister kinetochores not being attached to microtubules emanating from opposite poles. Here we describe a differential requirement for Sgo1 in the segregation of homologous chromosomes and sister chromatids. Sgo1 plays only a minor role in segregating homologous chromosomes at meiosis I. In contrast, Sgo1 is important to bias sister kinetochores toward biorientation. We suggest that Sgo1 acts at sister kinetochores to promote their biorientation.