PCR-based sex determination of cetaceans and dugong from the Indian seas
PCR-based sex determination of cetaceans and dugong from the Indian seas
复制标题
基于 PCR 的印度海鲸目动物和儒艮性别测定
DOI：
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发表时间：
2008
期刊：
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通讯作者：
M. Rajagopalan
中科院分区：
文献类型：
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作者：
P. Jayasankar;B. Anoop;M. Rajagopalan
一种基于从皮肤组织中提取的基因组DNA的PCR扩增的性别决定技术已经在鲸目动物和印度洋河豚样本中标准化。用特异的聚合酶链式反应(PCR)扩增出基因组中210-224个碱基对的Y染色体特异性区域(SRY或性别决定基因)(仅在男性)。同时用另一对引物同时扩增出ZFX/ZFY(分别位于X和Y染色体上的锌指蛋白基因)442-445个碱基的片段，作为性别确认的阳性对照。根据《印度野生动物法案》，对长吻海豚(Stenella Longirostris)、白头海豚(Stenella Tenuata)、宽吻海豚(Tursiops Aduncus)、印度-太平洋驼背海豚(Sousa Chinensis)、里索海豚(Grampus Griseus)、江豚(Neopo-caena phocaenoides)、抹香鲸(Physeter Maccephalus)、蓝鲸(Balaenoptera Musculus)、布赖德鲸(Balaenoptera Edeni)和独角鲸(Dugong du-gon)等濒危物种进行了标准化的性别鉴定。
A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act.