XTbx1 is a transcriptional activator involved in head and pharyngeal arch development in Xenopus laevis
XTbx1 is a transcriptional activator involved in head and pharyngeal arch development in Xenopus laevis
复制标题
DOI：
10.1002/dvdy.20276
复制
发表时间：
2005-04-01
影响因子：
2.5
通讯作者：
Scambler, PJ
中科院分区：
文献类型：
--
作者：
Ataliotis, P;Ivins, S;Scambler, PJ
小鼠和斑马鱼胚胎中咽弓衍生物的发育取决于转录因子 Tbx1 的活性。我们克隆了 Tbx1 (XTbx1) 的非洲爪蟾直系同源物，并表明其表达模式与其他脊椎动物物种相似。合子转录本在中囊胚转变后不久首先被检测到，并定位于中原肠胚阶段的假定中胚层。 XTbx1 表达持续存在于神经胚阶段的侧板中胚层中，并且从早期尾芽阶段开始在咽弓和耳囊中发现。我们证明 XTbx1 是一种转录激活剂，并且这种反式激活需要蛋白质的 C 端区域。 XTbx1 的显性干扰突变体破坏了非洲爪蟾头部结构和咽弓衍生物的发育。谱系标记揭示了有助于咽中胚层和 fgf8 表达的细胞中 XTbx1 功能的需要。 (c) 2005 年 Wiley-Liss, Inc.
The development of pharyngeal arch derivatives in mouse and zebrafish embryos depends on the activity of the transcription factor Tbx1. We cloned the Xenopus laevis orthologue of Tbx1 (XTbx1) and show that the pattern of expression is similar to that in other vertebrate species. Zygotic transcripts are first detected shortly after the mid-blastula transition and are localized to the presumptive mesoderm at mid-gastrula stages. XTbx1 expression persists in the lateral plate mesoderm at neurula stages and is found in the pharyngeal arches and otic vesicles from early tail bud stages onward. We demonstrate that XTbx1 is a transcriptional activator and that this trans-activation requires the C-terminal region of the protein. A dominant interfering mutant of XTbx1 disrupts the development of Xenopus head structures and pharyngeal arch derivatives. Lineage labeling reveals a requirement for XTbx1 function in cells that contribute to the pharyngeal mesoderm and for fgf8 expression. (c) 2005 Wiley-Liss, Inc.