Microinjection of cultured rat embryos: studies with retinol, 13-cis- and all-trans-retinoic acid.
Microinjection of cultured rat embryos: studies with retinol, 13-cis- and all-trans-retinoic acid.
复制标题
培养大鼠胚胎的显微注射：视黄醇、13-顺式和全反式视黄酸的研究。
DOI：
10.1002/tera.1420440308
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Kraft,JC
中科院分区：
文献类型：
--
作者：
Lee,QP;Juchau,MR;Kraft,JC
Retinol, all‐trans‐retinoic acid or 13‐cis‐retinoic acid were intraamniotically microinjected in rat embryos on day 10 of gestation and cultured until day 11.5. A comparison of the concentration–effect relationships of the retinoids showed that the dysmorphogenic effects were qualitatively similar for all three, but were elicited by a low concentration of all‐trans‐retinoic acid (250 ng/ml), a 6‐ to 7‐fold higher concentration of 13‐cis‐retinoic acid and an approximately 16‐fold higher concentration of retinol. After microinjection of 2,000 ng/ml of retinol, no dysmorphogenesis was observed but instead an increase in all growth parameters as compared to the controls.
