Somatostatin analogs inhibit somatostatin secretion from cultured hypothalamus cells.
Somatostatin analogs inhibit somatostatin secretion from cultured hypothalamus cells.
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生长抑素类似物抑制培养的下丘脑细胞分泌生长抑素。
DOI：
10.1159/000124011
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发表时间：
1984
影响因子：
4.1
通讯作者：
Vale,WW
中科院分区：
文献类型：
--
作者：
Peterfreund,RA;Vale,WW
在长期原代培养的胎鼠下丘脑细胞模型系统中，研究了生长抑素（SRIF）类似物对放射免疫测定SRIF（SRIF-LI）分泌的影响。这些实验利用了某些合成SRIF的生物活性，这些合成SRIF在我们的SRIF标准RIA中交叉反应很差。[DTrp 8]-SRIF-14、[DTrp 22]-SRIF-28和八肽des-AA 1、2、4、5、12、13[DTrp 8]-SRIF可抑制氨甲酰胆碱刺激的SRIF-LI分泌。合成类似物的有效浓度等于或低于InM。des-Trp 8-SRIF-14是一种在所有其他SRIF生物测定中无活性的类似物，在该测定系统中在高达10 nM的剂量下同样无效。通过洗涤除去肽后，肽处理的细胞和对照细胞对高钾缓冲液的去极化反应相似。数据表明SRIF类似物对下丘脑抑制SRIF-LI释放的直接作用，并且与内源性肽参与完整动物中其自身分泌的“超短环”负反馈控制的可能性一致。
The effect of somatostatin (SRIF) analogs on the secretion of radioimmunoassayable SRIF (SRIF-LI) was studied in the model system of fetal rat hypothalamus cells maintained in long-term, primary dispersed culture. These experiments exploited the biologic activity of certain synthetic SRIFs which cross-react poorly in our standard RIA for SRIF. Carbachol-stimulated SRIF-LI secretion was suppressed by [DTrp8]-SRIF-14, [DTrp22]-SRIF-28 and the octapeptide, des-AA1,2,4,5,12,13[DTrp8]-SRIF. Effective concentrations of synthetic analogs were at or below 1 nM. des-Trp8-SRIF-14, an analog inactive in all other SRIF biological assays, was similarly ineffective in this assay system at doses up to 10 nM. Following removal of peptide by washing, peptide-treated and control cells responded similarly to depolarization with high potassium buffer. The data demonstrate a direct action of SRIF analogs on the hypothalamus to suppress SRIF-LI release and are consistent with the possibility that an endogenous peptide participates in ‘ultrashortloop’ negative feedback control of its own secretion in the intact animal.