In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts.
In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts.
复制标题
使用 SP（P 物质）和 SDF（基质细胞衍生因子）-1 肽洗脱血管移植物进行原位血管再生
DOI：
10.1161/atvbaha.118.310934
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Kim SH
中科院分区：
文献类型：
--
作者：
Shafiq M;Zhang Q;Zhi D;Wang K;Kong D;Kim DH;Kim SH
补充数字内容可在文本中找到。本研究的目的是开发能够洗脱SDF（基质细胞衍生因子）-1α衍生肽和SP（P物质）的小直径血管移植物，用于原位血管再生。采用静电纺丝技术制备了含SP或SDF-1α衍生肽的聚己内酯（PCL）/胶原接枝物。SP和SDF-1α肽负载的移植物募集的间充质干细胞数量显著高于对照组。通过将PCL/胶原、SDF-1和SP移植物植入大鼠腹主动脉长达4周来评估其体内潜力。彩色多普勒和体视显微镜观察，所有移植物均保持通畅。宿主细胞浸润到移植物壁中，并且在肽洗脱移植物中形成新生内膜。SP移植物的管腔被鹅卵石样形态的内皮细胞覆盖，扫描电子显微镜观察到这些细胞沿血流方向伸长。此外，SDF-1α和SP移植物导致融合内皮的形成，如使用血管性血友病因子抗体的免疫荧光染色所评价的。SP和SDF-1α移植物还促进平滑肌细胞再生、内源性干细胞募集和血管形成，这在SP移植物中最为突出。炎症反应评价显示，3组在促炎巨噬细胞数量方面没有显著差异，而SP移植物显示促重塑巨噬细胞的数量显著高于对照组和SDF-1α移植物。SDF-1α和SP移植物可作为血管原位再生的候选材料，值得进一步研究。
Supplemental Digital Content is available in the text. The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell–derived factor)-1α–derived peptide and SP (substance P) for in situ vascular regeneration. Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α–derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.