Analysis of the effect of disturbed flow on monocytic adhesion to endothelial cells
Analysis of the effect of disturbed flow on monocytic adhesion to endothelial cells
复制标题
DOI：
10.1016/s0021-9290(03)00210-0
复制
发表时间：
2003-12-01
影响因子：
2.4
通讯作者：
Usami, S
中科院分区：
文献类型：
--
作者：
Chiu, JJ;Chen, CN;Usami, S
单核细胞优先与血管内皮细胞(ECs)黏附在动脉树分支和弯曲附近的区域，这表明血流动力学条件在单核细胞黏附中起着重要作用。本研究旨在阐明扰动流动对单核细胞与内皮细胞黏附的影响以及内皮细胞的黏附特性。我们首次应用微米分辨率粒子图像测速技术(MuPIV)分析了垂直阶梯流(VSF)室内的扰动流动特性。结果表明，在台阶和再附着点附近，单核细胞类似物THP-1细胞存在较高的近壁浓度和较长的停留时间。THP-1细胞与经肿瘤坏死因子α处理的内皮细胞在靠近台阶和再贴壁点的区域表现出显著的黏附，但与未刺激的内皮细胞几乎没有黏附。用抗细胞间黏附分子-1(ICAM-1)、血管黏附分子-1(VCAM-1)和E-选择素的抗体预先孵育经肿瘤坏死因子α处理的内皮细胞，可抑制THP-1的黏附；血管内皮细胞在VSF中预先暴露于扰动流中24 h后，其表面ICAM-I和E-选择素的表达显著增加，而VCAM-1的表达无明显变化，THP-1细胞的黏附能力明显增强。我们的研究结果表明，在动脉粥样硬化病变的流行区域，复杂的流动环境在调节血管内皮细胞的黏附特性以及随后的单核细胞黏附方面具有重要意义。(C)2003爱思唯尔有限公司。保留所有权利。
The preferential adhesion of monocytes to vascular endothelial cells (ECs) at regions near branches and curvatures of the arterial tree, where flow is disturbed, suggests that hemodynamic conditions play significant roles in monocyte adhesion. The present study aims to elucidate the effects of disturbed flow on monocyte adhesion to ECs and the adhesive properties of ECs. We applied, for the first time, the micron-resolution particle image velocimetry (muPIV) technique to analyze the characteristics of the disturbed flow produced in our vertical-step flow (VSF) chamber. The results demonstrated the existence of a higher near-wall concentration and a longer residence time of the monocytic analog THP-1 cells near the step and the reattachment point. THP-1 cells showed prominent adhesion to ECs pretreated with TNFalpha in the regions near the step and the reattachment point, but they showed virtually no adhesion to un-stimulated ECs. Pre-incubation of the TNFalpha-treated ECs with antibodies against intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), and E-selectin inhibited the THP-1 adhesion; the maximal inhibition was observed with a combination of these antibodies. Pre-exposure of ECs to disturbed flow in VSF for 24 h led to significant increases in their surface expressions of ICAM-I and E-selectin, but not VCAM-1, and in the adhesion of THP-1 cells. Our findings demonstrate the importance of complex flow environment in modulating the adhesive properties of vascular endothelium and consequently monocyte adhesion in regions of prevalence of atherosclerotic lesions. (C) 2003 Elsevier Ltd. All rights reserved.