Androgens upregulate aquaporin 9 expression in the prostate
Androgens upregulate aquaporin 9 expression in the prostate
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DOI：
10.1111/j.1442-2042.2008.02130.x
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Yokoyama, Masayoshi
中科院分区：
文献类型：
--
作者：
Wang, Jianbo;Tanji, Nozomu;Yokoyama, Masayoshi
目的：水通道蛋白（Aquaporins，AQP）作为一种选择性孔道，允许水、甘油等小分子溶质通过细胞膜。我们以前报道过AQP在人和大鼠的前列腺中都有表达。本研究探讨了雄激素依赖性的AQP 9在前列腺中的表达在体内和体外invival.Methods：大鼠腹侧前列腺组织标本和正常人前列腺上皮细胞系（PNT 2）。采用实时定量聚合酶链反应、免疫印迹和免疫组织化学方法检测AQP 9 mRNA和蛋白表达。通过手术去势、丙酸睾酮（5 μ g/g体重）或比卡鲁胺（20 μ g/g体重）治疗以及雄激素受体（AR）的核糖核酸干扰（RNAi）方法实现雄激素调节。将合成的小干扰RNA以10 nM的浓度转染到PNT 2中，并通过Western blotting分析评估RNAi效应。手术去势或比卡鲁胺治疗显著降低其表达。此外，去势后用丙酸睾酮处理使表达恢复到对照水平。结论：AQP 9在前列腺中的表达受雄激素的调控。
Objective: Aquaporins (AQP) function as selective pores allowing water, glycerol and other small solutes to pass through the cell membrane. We previously reported that AQP are expressed in the prostates of both humans and rats. The present study investigated the androgen-dependent expression of AQP9 in the prostate in vivo and in vitro.Methods: Rat ventral prostate tissue specimens and a normal human prostatic epithelial cell line (PNT2) were used. AQP9 messenger ribonucleic acid (mRNA) and protein expression were examined using real-time polymerase chain reaction, Western blotting and immunohistochemical methods. Androgen modulation was achieved by surgical castration, treatment with testosterone propionate (5 mu g/g bodyweight) or bicalutamide (20 mu g/g bodyweight), and the ribonucleic acid interference (RNAi) method of the androgen receptor (AR). The synthetic small interfering RNA was transfected into PNT2 at a concentration of 10 nM, and the RNAi effect was evaluated using a Western blotting analysis.Results: AQP9 mRNA and protein were expressed in the rat prostate. Surgical castration or bicalutamide treatment significantly decreased their expression. In addition, the treatment with testosterone propionate after castration restored the expression to the level of the controls. An RNAi experiment in PNT2 also decreased the expression.Conclusions: AQP9 expression in the prostate is controlled by androgens.