SLP-65: a new signaling component in B lymphocytes which requires expression of the antigen receptor for phosphorylation.
SLP-65: a new signaling component in B lymphocytes which requires expression of the antigen receptor for phosphorylation.
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DOI：
10.1084/jem.188.4.791
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发表时间：
1998-08-17
影响因子：
15.3
通讯作者：
Reth, M
中科院分区：
文献类型：
--
作者：
Wienands, J;Schweikert, J;Wollscheid, B;Jumaa, H;Nielsen, P J;Reth, M
B细胞抗原受体（BCR）由作为抗原结合亚基的膜结合免疫球蛋白（IG）分子和作为信号亚基的IG-α/IG-β异源二聚体组成。BCR信号转导涉及蛋白酪氨酸激酶（PTK）的激活和几种蛋白质的磷酸化，其中只有一些已被确定。这些蛋白质的磷酸化可以通过将B细胞暴露于抗原或酪氨酸磷酸酶抑制剂过钒酸盐/H2 O2来诱导。B细胞中最早的底物之一是一种65 kD的蛋白质，我们在此将其鉴定为B细胞接头蛋白。这种蛋白质被命名为SLP-65，是涉及Grb-2和Vav的信号复合物的一部分，并与T细胞受体的信号元件SLP-76具有同源性。在过钒酸盐/H2 O2刺激的细胞中，SLP-65仅在表达BCR时被磷酸化。这些数据表明，SLP-65是BCR换能器复合体的一部分。
The B cell antigen receptor (BCR) consists of the membrane-bound immunoglobulin (Ig) molecule as antigen-binding subunit and the Ig-α/Ig-β heterodimer as signaling subunit. BCR signal transduction involves activation of protein tyrosine kinases (PTKs) and phosphorylation of several proteins, only some of which have been identified. The phosphorylation of these proteins can be induced by exposure of B cells either to antigen or to the tyrosine phosphatase inhibitor pervanadate/H2O2. One of the earliest substrates in B cells is a 65-kD protein, which we identify here as a B cell adaptor protein. This protein, named SLP-65, is part of a signaling complex involving Grb-2 and Vav and shows homology to SLP-76, a signaling element of the T cell receptor. In pervanadate/H2O2-stimulated cells, SLP-65 becomes phosphorylated only upon expression of the BCR. These data suggest that SLP-65 is part of a BCR transducer complex.