CITRATE AND CONVERSION OF CARBOHYDRATE INTO FAT - REGULATION OF FATTY ACID SYNTHESIS BY RAT LIVER EXTRACTS
CITRATE AND CONVERSION OF CARBOHYDRATE INTO FAT - REGULATION OF FATTY ACID SYNTHESIS BY RAT LIVER EXTRACTS
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DOI：
10.1042/bj1050803
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发表时间：
1967-01-01
影响因子：
4.1
通讯作者：
LOWENSTEIN, JM
中科院分区：
文献类型：
--
作者：
FANG, M;LOWENSTEIN, JM
如果首先用柠檬酸盐激活酶系统，则从大鼠肝脏制备的无颗粒提取物的脂肪酸合成速率大大增加。活化程度以相互依赖的方式取决于柠檬酸盐浓度和活化时间。柠檬酸盐活化强烈依赖于温度。丙三羧酸酯可以代替柠檬酸盐作为活化剂，但其在测定中的存在抑制脂肪酸合成。Mg~（2+）离子可以在活化过程中取代柠檬酸盐，但不能在整个反应体系中取代柠檬酸盐。ATP阻止柠檬酸盐和Mg2+离子的活化作用。棕榈酰-DL-肉碱增加脂肪酸合成的速率。除了柠檬酸盐引起的激活外，这种类型的激活是快速的，不依赖于先前的孵育。棕榈酰辅酶A对脂肪酸合成的抑制作用可以通过棕榈酰-DL-肉碱或增加蛋白质浓度来预防。
The rate of fatty acid synthesis by particle-free extracts prepared from rat liver is increased greatly if the enzyme system is 1st activated with citrate. The extent of the activation depends on the citrate concentration and on the time of activation in an interdependent manner. Citrate activation is strongly dependent on temperature. Tricarballylate can replace citrate as an activator, but its presence in the assay inhibits fatty acid synthesis. Mg2+ ions can replace citrate in the activation but not in the complete reaction system. ATP prevents the activating effect of citrate and Mg2+ ions. The rate of fatty acid synthesis is increased by palmitoyl-DL-carnitine. This type of activation, additional to that caused by citrate, is rapid and does not depend on prior incubation. Inhibition of fatty acid synthesis by palmitoyl-CoA can be prevented by palmitoyl-DL-carnitine or by increasing the concentration of protein.