Isolation of a European bat lyssavirus type 2 from a Daubenton's bat in the United Kingdom
Isolation of a European bat lyssavirus type 2 from a Daubenton's bat in the United Kingdom
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DOI：
10.1136/vr.152.13.383
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发表时间：
2003-03-29
影响因子：
2.2
通讯作者：
Fooks, AR
中科院分区：
文献类型：
--
作者：
Johnson, N;Selden, D;Fooks, AR
欧洲蝙蝠狂犬病病毒2型（EBLV-2）曾于1996年6月在英国的一只蝙蝠中分离出来。2002年9月，在兰开夏郡发现的一只道氏鼠耳蝠（Myotis daughonii）在圈养期间出现了异常行为，包括无端的攻击。对蝙蝠的脑样本进行了溶血病毒属病毒检测，其中包括EBLV-2（基因型6）和经典狂犬病病毒（基因型1）。阳性荧光抗体检测证实其感染了狂犬病病毒，PCR和基因组测序鉴定该病毒为EBLV-2a。与基因型6的所有已发表序列的系统发育比较表明，它与英国以前的EBLV-2分离株密切相关，并建议与荷兰蝙蝠分离株有联系。从英格兰西海岸发现的蝙蝠中分离出EBLV-2提供了证据，表明这种病毒可能存在于英国Daughon的蝙蝠种群中，流行率较低。
European bat lyssavirus type 2 (EBLV-2) has been isolated once previously from a bat in the UK in June 1996. In September 2002, a Daubenton's bat (Myotis daubentonii) found in Lancashire developed abnormal behaviour, including unprovoked aggression, while it was in captivity. Brain samples from the bat were tested for virus of the Lyssovirus genus, which includes EBLV-2 (genotype 6), and classical rabies virus (genotype 1). A positive fluorescent antibody test confirmed that it was infected with a lyssavirus, and PCR and genomic sequencing identified the virus as an EBLV-2a. Phylogenetic comparisons with all the published sequences from genotype 6 showed that it was closely related to the previous isolate Of EBLV-2 in the UK and suggested links to isolates from bats in the Netherlands. The isolation of EBLV-2 from a bat found on the west coast of England provides evidence that this virus may be present within the UK Daubenton's bat population at a low prevalence level.