Cell type-specific genotoxic effects of intermittent extremely low-frequency electromagnetic fields
Cell type-specific genotoxic effects of intermittent extremely low-frequency electromagnetic fields
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DOI：
10.1016/j.mrgentox.2005.03.011
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发表时间：
2005-06-06
影响因子：
1.9
通讯作者：
Rüdiger, HW
中科院分区：
文献类型：
--
作者：
Ivancsits, S;Pilger, A;Rüdiger, HW
极低频电磁场（ELF-EMFs）对健康的不良影响是一个极具争议的问题。关于ELF-EMF的基因毒性潜力，文献中已经报道了相互矛盾的结果。为了测试这一争议是否反映了所检查的细胞靶点之间的差异，我们将来自不同组织的培养细胞暴露于间歇性的ELF-EMF （50 Hz正弦，1 mT）中1-24小时。碱性和中性彗星试验用于评估ELF-EMF诱导的DNA链断裂。我们可以鉴定出三种应答细胞（人类成纤维细胞、人类黑色素细胞、大鼠颗粒细胞）和三种非应答细胞类型（人类淋巴细胞、人类单核细胞、人类骨骼肌细胞），这表明了在研究ELF-EMF基因毒性效应时使用的细胞系统的重要性(c) 2005 Elsevier B.V.。
The issue of adverse health effects of extremely low-frequency electromagnetic fields (ELF-EMFs) is highly controversial. Contradictory results regarding the genotoxic potential of ELF-EMF have been reported in the literature, To test whether this controversy might reflect differences between the cellular targets examined we exposed Cultured cells derived from different tissues to an intermittent ELF-EMF (50 Hz sinusoidal, 1 mT) for 1-24 h. The alkaline and neutral comet assays were used to assess ELF-EMF-induced DNA strand breaks. We could identify three responder (human fibroblasts, human melanocytes, rat granulosa cells) and three non-responder cell types (human lymphocytes, human monocytes, human skeletal muscle cells), which points to the significance of the cell system used when investigating genotoxic effects of ELF-EMF (c) 2005 Elsevier B.V. All rights reserved.