Biological synthesis of high-conductive pili in aerobic bacterium Pseudomonas aeruginosa
Biological synthesis of high-conductive pili in aerobic bacterium Pseudomonas aeruginosa
复制标题
需氧菌铜绿假单胞菌高导菌毛的生物合成
DOI：
10.1007/s00253-018-9484-5
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发表时间：
2019-02-01
影响因子：
5
通讯作者：
Ma, Luyan Z.
中科院分区：
文献类型：
--
作者：
Liu, Xi;Wang, Shiwei;Ma, Luyan Z.
生物电纳米线作为生态材料在环境应用方面具有巨大潜力。许多细菌都可以表达 IV 型菌毛 (T4P)，这是由 PilA 组装而成的长蛋白纤维。硫还原地杆菌的 T4P 被称为微生物纳米线，但铜绿假单胞菌 (PaT4P) 的 T4P 被认为导电性较差。铜绿假单胞菌是一种需氧且具有电化学活性的细菌。已知其 T4P 负责表面附着、抽搐运动和生物膜形成。在这里，我们证明 PaT4P 在由仅含有 N 末端 61 个氨基酸的截短铜绿假单胞菌 PilA (PaPilA) 组装时具有高导电性。此外，增加PaPilA(1-61)中芳香族氨基酸的数量显着增强微生物燃料电池系统中菌毛的电导率和铜绿假单胞菌的生物电输出，表明PaT4P作为导电纳米材料的潜在应用。来自不同真细菌的 PilA 的 N 端区域高度保守，这意味着合成高导电微生物纳米线和增加微生物燃料电池生物电输出的通用方法。
Bioelectrical nanowires as ecomaterials have great potential on environmental applications. A wide range of bacteria can express type IV pili (T4P), which are long protein fibers assembled from PilA. The T4P of Geobacter sulfurreducens are well known as microbial nanowires, yet T4P of Pseudomonas aeruginosa (PaT4P) was believed to be poorly conductive. P. aeruginosa is an aerobic and electrochemically active bacterium. Its T4P have been known to be responsible for surface attachment, twitching motility and biofilm formation. Here, we show that PaT4P can be highly conductive while assembled by a truncated P. aeruginosa PilA (PaPilA) containing only N-terminus 61 amino acids. Furthermore, increasing the number of aromatic amino acids in the PaPilA(1-61) significantly enhances the conductivity of pili and the bioelectricity output of P. aeruginosa in microbial fuel cell system, suggesting a potential application of PaT4P as a conductive nanomaterial. The N-terminal region of PilA from diverse eubacteria is highly conserved, implying a general way to synthesize highly conductive microbial nanowires and to increase the bioelectricity output of microbial fuel cell.